Frequency alterations in key innate immune cell components in the peripheral blood of dengue patients detected by FACS analysis

Jih Jin Tsai, Yen Hua Jen, Jung San Chang, Hui Mien Hsiao, Sansanee Noisakran, Guey Chuen Perng

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

Dengue is a timing disease, and frequently dengue patients do not seek help until 2-3 days after prodrome. Thus, the innate immune parameters in dengue patients remain largely unexplored. A comprehensive FACS profiling of key innate immune cells in the peripheral blood of dengue patients was performed. Twenty-seven dengue patients varying in days of fever onset were enrolled and the majority of them had primary infection based on serological results. FACS panels were used to evaluate NK cells, platelet-leukocyte aggregates (PLA), inflammatory monocytes, and plasmacytoid (pDC) and myeloid dendritic cells (mDC). FACS results showed the following findings: (i) NK cells significantly dropped on day 5 after the onset of fever and gradually returned to normal within 2 weeks of illness; (ii) biphasic PLA were observed, maximum levels were reached on days 6-8 and 11-16, and platelet-monocyte aggregates (PMA) were the most frequent event; (iii) inflammatory monocytes were consistently lower on days 5-8 and gradually returned to the normal level in the second week of illness, and (iv) pDC reached the maximum level on day 5 and gradually declined to the baseline level after 1 week of illness. In contrast, mDC fluctuated somewhat during the first week of illness and returned to the baseline level in the second week of illness. The most interesting finding was the biphasic PLA, in particular the PMA. These innate immunological parameters could be a crucial factor dictating the complicated pathogenesis of dengue disease.

Original languageEnglish
Pages (from-to)530-540
Number of pages11
JournalJournal of Innate Immunity
Volume3
Issue number5
DOIs
Publication statusPublished - 2011 Aug 1

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy

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