Functional analysis of transcription factors in arabidopsis

Nobutaka Mitsuda, Masaru Ohme-Takagi

Research output: Contribution to journalReview articlepeer-review

207 Citations (Scopus)


Transcription factors (TFs) regulate the expression of genes at the transcriptional level. Modification of TF activity dynamically alters the transcriptome, which leads to metabolic and phenotypic changes. Thus, functional analysis of TFs using 'omics-based' methodologies is one of the most important areas of the post-genome era. In this mini-review, we present an overview of Arabidopsis TFs and introduce strategies for the functional analysis of plant TFs, which include both traditional and recently developed technologies. These strategies can be assigned to five categories: bioinformatic analysis; analysis of molecular function; expression analysis; phenotype analysis; and network analysis for the description of entire transcriptional regulatory networks.

Original languageEnglish
Pages (from-to)1232-1248
Number of pages17
JournalPlant and Cell Physiology
Issue number7
Publication statusPublished - 2009 Jul

All Science Journal Classification (ASJC) codes

  • Physiology
  • Plant Science
  • Cell Biology


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