Functional effects of let-7g expression in colon cancer metastasis

Che Mai Chang, Henry Sung Ching Wong, Chien Yu Huang, Wen Li Hsu, Zhi Feng Maio, Siou Jin Chiu, Yao Ting Tsai, Ben-Kuen Chen, Yu Jui Yvonne Wan, Jaw Yuan Wang, Wei Chiao Chang

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

MicroRNA regulation is crucial for gene expression and cell functions. It has been linked to tumorigenesis, development and metastasis in colorectal cancer (CRC). Recently, the let-7 family has been identified as a tumor suppressor in different types of cancers. However, the function of the let-7 family in CRC metastasis has not been fully investigated. Here, we focused on analyzing the role of let-7g in CRC. The Cancer Genome Atlas (TCGA) genomic datasets of CRC and detailed data from a Taiwanese CRC cohort were applied to study the expression pattern of let-7g. In addition, in vitro as well as in vivo studies have been performed to uncover the effects of let-7g on CRC. We found that the expression of let-7g was significantly lower in CRC specimens. Our results further supported the inhibitory effects of let-7g on CRC cell migration, invasion and extracellular calcium influx through store-operated calcium channels. We report a critical role for let-7g in the pathogenesis of CRC and suggest let-7g as a potential therapeutic target for CRC treatment.

Original languageEnglish
Article number489
JournalCancers
Volume11
Issue number4
DOIs
Publication statusPublished - 2019 Apr 1

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Colonic Neoplasms
Colorectal Neoplasms
Neoplasm Metastasis
Neoplasms
Atlases
Calcium Channels
MicroRNAs
Cell Movement
Carcinogenesis
Genome
Calcium
Gene Expression

All Science Journal Classification (ASJC) codes

  • Oncology
  • Cancer Research

Cite this

Chang, C. M., Wong, H. S. C., Huang, C. Y., Hsu, W. L., Maio, Z. F., Chiu, S. J., ... Chang, W. C. (2019). Functional effects of let-7g expression in colon cancer metastasis. Cancers, 11(4), [489]. https://doi.org/10.3390/cancers11040489
Chang, Che Mai ; Wong, Henry Sung Ching ; Huang, Chien Yu ; Hsu, Wen Li ; Maio, Zhi Feng ; Chiu, Siou Jin ; Tsai, Yao Ting ; Chen, Ben-Kuen ; Wan, Yu Jui Yvonne ; Wang, Jaw Yuan ; Chang, Wei Chiao. / Functional effects of let-7g expression in colon cancer metastasis. In: Cancers. 2019 ; Vol. 11, No. 4.
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abstract = "MicroRNA regulation is crucial for gene expression and cell functions. It has been linked to tumorigenesis, development and metastasis in colorectal cancer (CRC). Recently, the let-7 family has been identified as a tumor suppressor in different types of cancers. However, the function of the let-7 family in CRC metastasis has not been fully investigated. Here, we focused on analyzing the role of let-7g in CRC. The Cancer Genome Atlas (TCGA) genomic datasets of CRC and detailed data from a Taiwanese CRC cohort were applied to study the expression pattern of let-7g. In addition, in vitro as well as in vivo studies have been performed to uncover the effects of let-7g on CRC. We found that the expression of let-7g was significantly lower in CRC specimens. Our results further supported the inhibitory effects of let-7g on CRC cell migration, invasion and extracellular calcium influx through store-operated calcium channels. We report a critical role for let-7g in the pathogenesis of CRC and suggest let-7g as a potential therapeutic target for CRC treatment.",
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Chang, CM, Wong, HSC, Huang, CY, Hsu, WL, Maio, ZF, Chiu, SJ, Tsai, YT, Chen, B-K, Wan, YJY, Wang, JY & Chang, WC 2019, 'Functional effects of let-7g expression in colon cancer metastasis', Cancers, vol. 11, no. 4, 489. https://doi.org/10.3390/cancers11040489

Functional effects of let-7g expression in colon cancer metastasis. / Chang, Che Mai; Wong, Henry Sung Ching; Huang, Chien Yu; Hsu, Wen Li; Maio, Zhi Feng; Chiu, Siou Jin; Tsai, Yao Ting; Chen, Ben-Kuen; Wan, Yu Jui Yvonne; Wang, Jaw Yuan; Chang, Wei Chiao.

In: Cancers, Vol. 11, No. 4, 489, 01.04.2019.

Research output: Contribution to journalArticle

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AU - Maio, Zhi Feng

AU - Chiu, Siou Jin

AU - Tsai, Yao Ting

AU - Chen, Ben-Kuen

AU - Wan, Yu Jui Yvonne

AU - Wang, Jaw Yuan

AU - Chang, Wei Chiao

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AB - MicroRNA regulation is crucial for gene expression and cell functions. It has been linked to tumorigenesis, development and metastasis in colorectal cancer (CRC). Recently, the let-7 family has been identified as a tumor suppressor in different types of cancers. However, the function of the let-7 family in CRC metastasis has not been fully investigated. Here, we focused on analyzing the role of let-7g in CRC. The Cancer Genome Atlas (TCGA) genomic datasets of CRC and detailed data from a Taiwanese CRC cohort were applied to study the expression pattern of let-7g. In addition, in vitro as well as in vivo studies have been performed to uncover the effects of let-7g on CRC. We found that the expression of let-7g was significantly lower in CRC specimens. Our results further supported the inhibitory effects of let-7g on CRC cell migration, invasion and extracellular calcium influx through store-operated calcium channels. We report a critical role for let-7g in the pathogenesis of CRC and suggest let-7g as a potential therapeutic target for CRC treatment.

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Chang CM, Wong HSC, Huang CY, Hsu WL, Maio ZF, Chiu SJ et al. Functional effects of let-7g expression in colon cancer metastasis. Cancers. 2019 Apr 1;11(4). 489. https://doi.org/10.3390/cancers11040489