Functional expression of a vertebrate inwardly rectifying K+ channel in yeast

Weimin Tang, Abdul Ruknudin, Wen Pin Yang, Shyh-Yu Shaw, Aron Knickerbocker, Stephen Kurtz

Research output: Contribution to journalArticle

57 Citations (Scopus)

Abstract

We describe the expression of gpIRK1, an inwardly rectifying K+ channel obtained from guinea pig cardiac cDNA. gpIRK1 is a homologue of the mouse IRK1 channel identified in macrophage cells. Expression of gpIRK1 in Xenopus oocytes produces inwardly rectifying K+ current, similar to the cardiac inward rectifier current I(K1). This current is blocked by external Ba2+ and Cs+. Plasmids containing the gpIRK1 coding region under the transcriptional control of constitutive (PGK) or inducible (GAL) promoters were constructed for expression in Saccharomyces cerevisiae. Several observations suggest that gpIRK1 forms functional ion channels when expressed in yeast. gpIRK1 complements a trk1Δ trk2Δ strain, which is defective in potassium uptake. Expression of gpIRK1 in this mutant restores growth on low potassium media. Growth dependent on gpIRK1 is inhibited by external Cs+. The strain expressing gpIRK1 provides a versatile genetic system for studying the assembly and composition of inwardly rectifying K+ channels.

Original languageEnglish
Pages (from-to)1231-1240
Number of pages10
JournalMolecular Biology of the Cell
Volume6
Issue number9
DOIs
Publication statusPublished - 1995 Jan 1

Fingerprint

Inwardly Rectifying Potassium Channel
Vertebrates
Yeasts
Potassium
Growth
Xenopus
Ion Channels
Oocytes
Saccharomyces cerevisiae
Guinea Pigs
Plasmids
Complementary DNA
Macrophages

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Cell Biology

Cite this

Tang, Weimin ; Ruknudin, Abdul ; Yang, Wen Pin ; Shaw, Shyh-Yu ; Knickerbocker, Aron ; Kurtz, Stephen. / Functional expression of a vertebrate inwardly rectifying K+ channel in yeast. In: Molecular Biology of the Cell. 1995 ; Vol. 6, No. 9. pp. 1231-1240.
@article{a54c206a4c94413ba9f3f5e87c5f9d4a,
title = "Functional expression of a vertebrate inwardly rectifying K+ channel in yeast",
abstract = "We describe the expression of gpIRK1, an inwardly rectifying K+ channel obtained from guinea pig cardiac cDNA. gpIRK1 is a homologue of the mouse IRK1 channel identified in macrophage cells. Expression of gpIRK1 in Xenopus oocytes produces inwardly rectifying K+ current, similar to the cardiac inward rectifier current I(K1). This current is blocked by external Ba2+ and Cs+. Plasmids containing the gpIRK1 coding region under the transcriptional control of constitutive (PGK) or inducible (GAL) promoters were constructed for expression in Saccharomyces cerevisiae. Several observations suggest that gpIRK1 forms functional ion channels when expressed in yeast. gpIRK1 complements a trk1Δ trk2Δ strain, which is defective in potassium uptake. Expression of gpIRK1 in this mutant restores growth on low potassium media. Growth dependent on gpIRK1 is inhibited by external Cs+. The strain expressing gpIRK1 provides a versatile genetic system for studying the assembly and composition of inwardly rectifying K+ channels.",
author = "Weimin Tang and Abdul Ruknudin and Yang, {Wen Pin} and Shyh-Yu Shaw and Aron Knickerbocker and Stephen Kurtz",
year = "1995",
month = "1",
day = "1",
doi = "10.1091/mbc.6.9.1231",
language = "English",
volume = "6",
pages = "1231--1240",
journal = "Molecular Biology of the Cell",
issn = "1059-1524",
publisher = "American Society for Cell Biology",
number = "9",

}

Tang, W, Ruknudin, A, Yang, WP, Shaw, S-Y, Knickerbocker, A & Kurtz, S 1995, 'Functional expression of a vertebrate inwardly rectifying K+ channel in yeast', Molecular Biology of the Cell, vol. 6, no. 9, pp. 1231-1240. https://doi.org/10.1091/mbc.6.9.1231

Functional expression of a vertebrate inwardly rectifying K+ channel in yeast. / Tang, Weimin; Ruknudin, Abdul; Yang, Wen Pin; Shaw, Shyh-Yu; Knickerbocker, Aron; Kurtz, Stephen.

In: Molecular Biology of the Cell, Vol. 6, No. 9, 01.01.1995, p. 1231-1240.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Functional expression of a vertebrate inwardly rectifying K+ channel in yeast

AU - Tang, Weimin

AU - Ruknudin, Abdul

AU - Yang, Wen Pin

AU - Shaw, Shyh-Yu

AU - Knickerbocker, Aron

AU - Kurtz, Stephen

PY - 1995/1/1

Y1 - 1995/1/1

N2 - We describe the expression of gpIRK1, an inwardly rectifying K+ channel obtained from guinea pig cardiac cDNA. gpIRK1 is a homologue of the mouse IRK1 channel identified in macrophage cells. Expression of gpIRK1 in Xenopus oocytes produces inwardly rectifying K+ current, similar to the cardiac inward rectifier current I(K1). This current is blocked by external Ba2+ and Cs+. Plasmids containing the gpIRK1 coding region under the transcriptional control of constitutive (PGK) or inducible (GAL) promoters were constructed for expression in Saccharomyces cerevisiae. Several observations suggest that gpIRK1 forms functional ion channels when expressed in yeast. gpIRK1 complements a trk1Δ trk2Δ strain, which is defective in potassium uptake. Expression of gpIRK1 in this mutant restores growth on low potassium media. Growth dependent on gpIRK1 is inhibited by external Cs+. The strain expressing gpIRK1 provides a versatile genetic system for studying the assembly and composition of inwardly rectifying K+ channels.

AB - We describe the expression of gpIRK1, an inwardly rectifying K+ channel obtained from guinea pig cardiac cDNA. gpIRK1 is a homologue of the mouse IRK1 channel identified in macrophage cells. Expression of gpIRK1 in Xenopus oocytes produces inwardly rectifying K+ current, similar to the cardiac inward rectifier current I(K1). This current is blocked by external Ba2+ and Cs+. Plasmids containing the gpIRK1 coding region under the transcriptional control of constitutive (PGK) or inducible (GAL) promoters were constructed for expression in Saccharomyces cerevisiae. Several observations suggest that gpIRK1 forms functional ion channels when expressed in yeast. gpIRK1 complements a trk1Δ trk2Δ strain, which is defective in potassium uptake. Expression of gpIRK1 in this mutant restores growth on low potassium media. Growth dependent on gpIRK1 is inhibited by external Cs+. The strain expressing gpIRK1 provides a versatile genetic system for studying the assembly and composition of inwardly rectifying K+ channels.

UR - http://www.scopus.com/inward/record.url?scp=0029145135&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0029145135&partnerID=8YFLogxK

U2 - 10.1091/mbc.6.9.1231

DO - 10.1091/mbc.6.9.1231

M3 - Article

VL - 6

SP - 1231

EP - 1240

JO - Molecular Biology of the Cell

JF - Molecular Biology of the Cell

SN - 1059-1524

IS - 9

ER -