Functional recoveries of sciatic nerve regeneration by combining chitosan-coated conduit and neurosphere cells induced from adipose-derived stem cells

Yuan-Yu Hsueh, Ya Ju Chang, Tzu Chieh Huang, Shih Chen Fan, Duo Hsiang Wang, Jia-Jin Chen, Chia-Ching Wu, Sheng Che Lin

Research output: Contribution to journalArticle

48 Citations (Scopus)

Abstract

Suboptimal repair occurs in a peripheral nerve gap, which can be partially restored by bridging the gap with various biosynthetic conduits or cell-based therapy. In this study, we developed a combination of chitosan coating approach to induce neurosphere cells from human adipose-derived stem cells (ASCs) on chitosan-coated plate and then applied these cells to the interior of a chitosan-coated silicone tube to bridge a 10-mm gap in a rat sciatic nerve. Myelin sheath degeneration and glial scar formation were discovered in the nerve bridged by the silicone conduit. By using a single treatment of chitosan-coated conduit or neurosphere cell therapy, the nerve gap was partially recovered after 6 weeks of surgery. Substantial improvements in nerve regeneration were achieved by combining neurosphere cells and chitosan-coated conduit based on the increase of myelinated axons density and myelin thickness, gastrocnemius muscle weight and muscle fiber diameter, and step and stride lengths from gait analysis. High expressions of interleukin-1β and leukotriene B4 receptor 1 in the intra-neural scarring caused by using silicone conduits revealed that the inflammatory mechanism can be inhibited when the conduit is coated with chitosan. This study demonstrated that the chitosan-coated surface performs multiple functions that can be used to induce neurosphere cells from ASCs and to facilitate nerve regeneration in combination with a cells-assisted coated conduit.

Original languageEnglish
Pages (from-to)2234-2244
Number of pages11
JournalBiomaterials
Volume35
Issue number7
DOIs
Publication statusPublished - 2014 Feb 1

Fingerprint

Nerve Regeneration
Chitosan
Sciatic Nerve
Stem cells
Stem Cells
Recovery
Silicones
Myelin Sheath
Cell- and Tissue-Based Therapy
Cicatrix
Muscle
Leukotriene B4 Receptors
Gait analysis
Interleukin-1
Gait
Peripheral Nerves
Neuroglia
Surgery
Axons
Rats

All Science Journal Classification (ASJC) codes

  • Bioengineering
  • Ceramics and Composites
  • Biophysics
  • Biomaterials
  • Mechanics of Materials

Cite this

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title = "Functional recoveries of sciatic nerve regeneration by combining chitosan-coated conduit and neurosphere cells induced from adipose-derived stem cells",
abstract = "Suboptimal repair occurs in a peripheral nerve gap, which can be partially restored by bridging the gap with various biosynthetic conduits or cell-based therapy. In this study, we developed a combination of chitosan coating approach to induce neurosphere cells from human adipose-derived stem cells (ASCs) on chitosan-coated plate and then applied these cells to the interior of a chitosan-coated silicone tube to bridge a 10-mm gap in a rat sciatic nerve. Myelin sheath degeneration and glial scar formation were discovered in the nerve bridged by the silicone conduit. By using a single treatment of chitosan-coated conduit or neurosphere cell therapy, the nerve gap was partially recovered after 6 weeks of surgery. Substantial improvements in nerve regeneration were achieved by combining neurosphere cells and chitosan-coated conduit based on the increase of myelinated axons density and myelin thickness, gastrocnemius muscle weight and muscle fiber diameter, and step and stride lengths from gait analysis. High expressions of interleukin-1β and leukotriene B4 receptor 1 in the intra-neural scarring caused by using silicone conduits revealed that the inflammatory mechanism can be inhibited when the conduit is coated with chitosan. This study demonstrated that the chitosan-coated surface performs multiple functions that can be used to induce neurosphere cells from ASCs and to facilitate nerve regeneration in combination with a cells-assisted coated conduit.",
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Functional recoveries of sciatic nerve regeneration by combining chitosan-coated conduit and neurosphere cells induced from adipose-derived stem cells. / Hsueh, Yuan-Yu; Chang, Ya Ju; Huang, Tzu Chieh; Fan, Shih Chen; Wang, Duo Hsiang; Chen, Jia-Jin; Wu, Chia-Ching; Lin, Sheng Che.

In: Biomaterials, Vol. 35, No. 7, 01.02.2014, p. 2234-2244.

Research output: Contribution to journalArticle

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AU - Wang, Duo Hsiang

AU - Chen, Jia-Jin

AU - Wu, Chia-Ching

AU - Lin, Sheng Che

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