@article{b3ac514d3ea94e07b8709b5a1ca32b2b,
title = "Functional Relevance of Interleukin-1 Receptor Inter-domain Flexibility for Cytokine Binding and Signaling",
abstract = "The IL-1 receptors are responsible for transmitting signaling in immune responses. Ge et al. investigated the effect of a common D2/D3 linker within IL-1 receptors and revealed the functional role of inherent inter-domain flexibility in ligand recognition and signal transduction.",
author = "Jiwan Ge and Remesh, {Soumya G.} and Michal Hammel and Si Pan and Mahan, {Andrew D.} and Shuying Wang and Xinquan Wang",
note = "Funding Information: We sincerely thank Professors Xianyang Fang (Tsinghua University), Haipeng Gong (Tsinghua University), and Xinqi Gong (Renmin University), and Dr. Meng Ke for helpful discussions. This work was conducted at the Advanced Light Source (ALS), a national user facility operated by Lawrence Berkeley National Laboratory on behalf of the Department of Energy, Office of Basic Energy Sciences, through the Integrated Diffraction Analysis Technologies (IDAT) program, supported by DOE Office of Biological and Environmental Research. Additional support comes from the National Institutes of Health project ALS-ENABLE (P30 GM124169) and a High-End Instrumentation grant S10OD018483. We thank Jinquan Luo of Janssen R&D, who supported this work, for collecting and analyzing mass spectroscopic data. This work was supported by the National Natural Science Foundation of China (31470751 and U1405228) and the National Key Plan for Scientific Research and Development of China (2016YFD0500307) to X.W. and also supported by the Ministry of Science and Technology, China (103-2311-B-006-006 and 104-2320-B-006-050) to S.W. Protein expression was performed by J.G. and S.P. SPR analysis, IL-1RAcP knockout cell line construction, and dual-luciferase reporter assay were performed by J.G. SAXS data collection was performed by M.H. and S.W. Solution structure modeling was performed by S.G.R. M.H. and J.G. Mass spectroscopy was performed by A.D.M. S.W. and X.W. J.G. prepared the manuscript. M.H. and S.G.R. revised the manuscript. X.W. and Y.W. were responsible for project direction. All authors reviewed the results and approved the final version of the manuscript. The authors declare no competing interests. Funding Information: We sincerely thank Professors Xianyang Fang (Tsinghua University), Haipeng Gong (Tsinghua University), and Xinqi Gong (Renmin University), and Dr. Meng Ke for helpful discussions. This work was conducted at the Advanced Light Source (ALS), a national user facility operated by Lawrence Berkeley National Laboratory on behalf of the Department of Energy , Office of Basic Energy Sciences , through the Integrated Diffraction Analysis Technologies (IDAT) program, supported by DOE Office of Biological and Environmental Research . Additional support comes from the National Institutes of Health project ALS-ENABLE ( P30 GM124169 ) and a High-End Instrumentation grant S10OD018483 . We thank Jinquan Luo of Janssen R&D , who supported this work, for collecting and analyzing mass spectroscopic data. This work was supported by the National Natural Science Foundation of China ( 31470751 and U1405228 ) and the National Key Plan for Scientific Research and Development of China ( 2016YFD0500307 ) to X.W., and also supported by the Ministry of Science and Technology , China ( 103-2311-B-006-006 and 104-2320-B-006-050 ) to S.W. Publisher Copyright: {\textcopyright} 2019 Elsevier Ltd",
year = "2019",
month = aug,
day = "6",
doi = "10.1016/j.str.2019.05.011",
language = "English",
volume = "27",
pages = "1296--1307.e5",
journal = "Structure with Folding & design",
issn = "0969-2126",
publisher = "Cell Press",
number = "8",
}