TY - GEN
T1 - Functionalized bio-nanoparticles in immunosensing using surface-enhanced raman scattering methods
AU - Lin, Chi Chang
AU - Yu, Chen Yuan
AU - Yang, Ying Mei
AU - Chen, Yan Fu
AU - Chang, Hsien Chang
PY - 2009
Y1 - 2009
N2 - An unique, sensitive, and highly specific immunoassay system using gold nanoparticles and Surface-Enhanced Raman Spectroscopy (SERS) is described. To demonstrate the analytical capabilities of the new technique, Raman reporter (DTNB) that is coimmobilized with biospecific anti-protein A on gold nanoparticles (AuNP). Anti-protein A-AuNP-DTNB, which combing both electromagnetic field and chemical enhancement, was used to exploits the SERS-derived signal. A dynamic range of 2-3 orders of magnitude and 1-10 pg/mL of detection limitation of protein A were achieved. The results indicated this new technique could be used in clinical diagnostic applications with fast, high sensitivity and highthroughput screening of antibodies.
AB - An unique, sensitive, and highly specific immunoassay system using gold nanoparticles and Surface-Enhanced Raman Spectroscopy (SERS) is described. To demonstrate the analytical capabilities of the new technique, Raman reporter (DTNB) that is coimmobilized with biospecific anti-protein A on gold nanoparticles (AuNP). Anti-protein A-AuNP-DTNB, which combing both electromagnetic field and chemical enhancement, was used to exploits the SERS-derived signal. A dynamic range of 2-3 orders of magnitude and 1-10 pg/mL of detection limitation of protein A were achieved. The results indicated this new technique could be used in clinical diagnostic applications with fast, high sensitivity and highthroughput screening of antibodies.
UR - http://www.scopus.com/inward/record.url?scp=70349679325&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=70349679325&partnerID=8YFLogxK
U2 - 10.1109/NEMS.2009.5068714
DO - 10.1109/NEMS.2009.5068714
M3 - Conference contribution
AN - SCOPUS:70349679325
SN - 9781424446308
T3 - 4th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2009
SP - 877
EP - 880
BT - 4th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2009
T2 - 4th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2009
Y2 - 5 January 2009 through 8 January 2009
ER -