Furosemide: A specific inhibitor of Pi transport across the plasma membrane of plant cells

Kiyoshi Okihara, Tetsuro Mimura, Seiichiro Kiyota, Katsuhiro Sakano

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9 Citations (Scopus)


A search was made for inhibitors of Pi uptake that act directly on the Pi transporter in the plasma membranes of Catharanthus roseus cells to inhibit Pi uptake without inhibition of proton pumping. Using standard electrodes, we monitored changes in pH and in the concentration of K+ ions, as well as the rate of Pi uptake, when an inhibitor to be tested was applied to the cells in unbuffered medium. A9C (28 μM), a blocker of anion channels, inhibited Pi uptake but it also inhibited the proton pump. However, a structurally similar inhibitor, furosemide, inhibited Pi uptake without inhibiting proton pumping.It is suggested that the carboxylic group of these inhibitors interacts with the Pi-binding site (probably an amino group) of the Pi transporter in the plasma membrane and that the hydrophobic structure of these inhibitors facilitates their accumulation in the plasma membrane.

Original languageEnglish
Pages (from-to)53-58
Number of pages6
JournalPlant and Cell Physiology
Issue number1
Publication statusPublished - 1995 Jan

All Science Journal Classification (ASJC) codes

  • Physiology
  • Plant Science
  • Cell Biology


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