Fusion protein of the hyaluronan binding domain from human TSG-6 with luciferase for assay of hyaluronan

Te Sheng Chang; Hisao Ming Wan; Chien Chiang Chen; R. Giridhar; Wen Teng Wu

doi:10.1023/A:1024178110310

Fusion protein of the hyaluronan binding domain from human TSG-6 with luciferase for assay of hyaluronan

Te Sheng Chang, Hisao Ming Wan, Chien Chiang Chen, R. Giridhar, Wen Teng Wu

College of Engineering

Research output: Contribution to journal › Article › peer-review

2 Citations (Scopus)

Abstract

The gene expression plasmid, pET-Lmluc, for the fusion protein of the hyaluronan binding domain from human TSG-6 [product of tumor necrosis factor (TNF)-stimulated gene-6] and luciferase from Renilla reniformis was constructed. The fused gene was expressed in Escherichia coli and the resulted insoluble Lm-luc fusion protein was purified and refolded to recover both the hyaluronan binding capability and the luciferase activity. Hyaluronan as low as 1 ng ml^-1 was detected by using the indirect enzymatic immunological assay with the refolded Lm-luc fusion protein.

Original language	English
Pages (from-to)	1037-1040
Number of pages	4
Journal	Biotechnology Letters
Volume	25
Issue number	13
DOIs	https://doi.org/10.1023/A:1024178110310
Publication status	Published - 2003 Jul

All Science Journal Classification (ASJC) codes

Biotechnology
Bioengineering
Applied Microbiology and Biotechnology

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10.1023/A:1024178110310

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abstract = "The gene expression plasmid, pET-Lmluc, for the fusion protein of the hyaluronan binding domain from human TSG-6 [product of tumor necrosis factor (TNF)-stimulated gene-6] and luciferase from Renilla reniformis was constructed. The fused gene was expressed in Escherichia coli and the resulted insoluble Lm-luc fusion protein was purified and refolded to recover both the hyaluronan binding capability and the luciferase activity. Hyaluronan as low as 1 ng ml-1 was detected by using the indirect enzymatic immunological assay with the refolded Lm-luc fusion protein.",

author = "Chang, {Te Sheng} and Wan, {Hisao Ming} and Chen, {Chien Chiang} and R. Giridhar and Wu, {Wen Teng}",

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T1 - Fusion protein of the hyaluronan binding domain from human TSG-6 with luciferase for assay of hyaluronan

AU - Chang, Te Sheng

AU - Wan, Hisao Ming

AU - Chen, Chien Chiang

AU - Giridhar, R.

AU - Wu, Wen Teng

PY - 2003/7

Y1 - 2003/7

N2 - The gene expression plasmid, pET-Lmluc, for the fusion protein of the hyaluronan binding domain from human TSG-6 [product of tumor necrosis factor (TNF)-stimulated gene-6] and luciferase from Renilla reniformis was constructed. The fused gene was expressed in Escherichia coli and the resulted insoluble Lm-luc fusion protein was purified and refolded to recover both the hyaluronan binding capability and the luciferase activity. Hyaluronan as low as 1 ng ml-1 was detected by using the indirect enzymatic immunological assay with the refolded Lm-luc fusion protein.

AB - The gene expression plasmid, pET-Lmluc, for the fusion protein of the hyaluronan binding domain from human TSG-6 [product of tumor necrosis factor (TNF)-stimulated gene-6] and luciferase from Renilla reniformis was constructed. The fused gene was expressed in Escherichia coli and the resulted insoluble Lm-luc fusion protein was purified and refolded to recover both the hyaluronan binding capability and the luciferase activity. Hyaluronan as low as 1 ng ml-1 was detected by using the indirect enzymatic immunological assay with the refolded Lm-luc fusion protein.

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JO - Biotechnology Letters

JF - Biotechnology Letters

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Fusion protein of the hyaluronan binding domain from human TSG-6 with luciferase for assay of hyaluronan

Abstract

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