Galectin-1 accelerates wound healing by regulating the neuropilin-1/Smad3/NOX4 pathway and ROS production in myofibroblasts

Yueh Te Lin, Jhih Sian Chen, Ming Heng Wu, I. Shan Hsieh, Chen Hsien Liang, Cheng Lung Hsu, Tse-Ming Hong, Yuh-Ling Chen

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

Myofibroblasts have a key role in wound healing by secreting growth factors and chemoattractants to create new substrates and proteins in the extracellular matrix. We have found that galectin-1, a β-galactose-binding lectin involved in many physiological functions, induces myofibroblast activation; however, the mechanism remains unclear. Here, we reveal that galectin-1-null (Lgals1 -/-) mice exhibited a delayed cutaneous wound healing response. Galectin-1 induced myofibroblast activation, migration, and proliferation by triggering intracellular reactive oxygen species (ROS) production. A ROS-producing protein, NADPH oxidase 4 (NOX4), was upregulated by galectin-1 through the neuropilin-1/Smad3 signaling pathway in myofibroblasts. Subcutaneous injection of galectin-1 into wound areas accelerated the healing of general and pathological (streptozotocin-induced diabetes mellitus) wounds and decreased the mortality of diabetic mice with skin wounds. These findings indicate that galectin-1 is a key regulator of wound repair that has therapeutic potential for pathological or imperfect wound healing.

Original languageEnglish
Pages (from-to)258-268
Number of pages11
JournalJournal of Investigative Dermatology
Volume135
Issue number1
DOIs
Publication statusPublished - 2015 Jan 1

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Neuropilin-1
Galectin 1
Myofibroblasts
NADPH Oxidase
Wound Healing
Reactive Oxygen Species
Wounds and Injuries
Chemical activation
Galectins
Skin
Experimental Diabetes Mellitus
Extracellular Matrix Proteins
Chemotactic Factors
Subcutaneous Injections
Streptozocin
Medical problems
Intercellular Signaling Peptides and Proteins
Diabetes Mellitus
Proteins
Repair

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Dermatology
  • Cell Biology

Cite this

Lin, Yueh Te ; Chen, Jhih Sian ; Wu, Ming Heng ; Hsieh, I. Shan ; Liang, Chen Hsien ; Hsu, Cheng Lung ; Hong, Tse-Ming ; Chen, Yuh-Ling. / Galectin-1 accelerates wound healing by regulating the neuropilin-1/Smad3/NOX4 pathway and ROS production in myofibroblasts. In: Journal of Investigative Dermatology. 2015 ; Vol. 135, No. 1. pp. 258-268.
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Galectin-1 accelerates wound healing by regulating the neuropilin-1/Smad3/NOX4 pathway and ROS production in myofibroblasts. / Lin, Yueh Te; Chen, Jhih Sian; Wu, Ming Heng; Hsieh, I. Shan; Liang, Chen Hsien; Hsu, Cheng Lung; Hong, Tse-Ming; Chen, Yuh-Ling.

In: Journal of Investigative Dermatology, Vol. 135, No. 1, 01.01.2015, p. 258-268.

Research output: Contribution to journalArticle

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