GEF-H1 controls focal adhesion signaling that regulates mesenchymal stem cell lineage commitment

I. Husan Huang, Cheng Te Hsiao, Jui Chung Wu, Rong Fong Shen, Ching Yi Liu, Yang Kao Wang, Yu Chen Chen, Chi Ming Huang, Juan C. del Álamo, Zee Fen Chang, Ming Jer Tang, Kay Hooi Khoo, Jean Cheng Kuo

Research output: Contribution to journalArticlepeer-review

31 Citations (Scopus)


Focal adhesions (FAs) undergo maturation that culminates in size and composition changes that modulate adhesion, cytoskeleton remodeling and differentiation. Although it is well recognized that stimuli for osteogenesis of mesenchymal stem cells (MSCs) drive FA maturation, actin organization and stress fiber polarization, the extent to which FA-mediated signals regulated by the FA protein composition specifies MSC commitment remains largely unknown. Here, we demonstrate that, upon dexamethasone (osteogenic induction) treatment, guanine nucleotide exchange factor H1 (GEF-H1, also known as Rho guanine nucleotide exchange factor 2, encoded by ARHGEF2) is significantly enriched in FAs. Perturbation of GEF-H1 inhibits FA formation, anisotropic stress fiber orientation and MSC osteogenesis in an actomyosin-contractility-independent manner. To determine the role of GEF-H1 in MSC osteogenesis, we explore the GEF-H1-modulated FA proteome that reveals non-muscle myosin-II heavy chain-B (NMIIB, also known as myosin-10, encoded by MYH10) as a target of GEF-H1 in FAs. Inhibition of targeting NMIIB into FAs suppresses FA formation, stress fiber polarization, cell stiffness and osteogenic commitments in MSCs. Our data demonstrate a role for FA signaling in specifying MSC commitment.

Original languageEnglish
Pages (from-to)4186-4200
Number of pages15
JournalJournal of Cell Science
Issue number19
Publication statusPublished - 2014

All Science Journal Classification (ASJC) codes

  • Cell Biology


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