TY - JOUR
T1 - Gene signatures of SARS-CoV/SARS-CoV-2-infected ferret lungs in short- and long-term models
AU - Liu, Hsin Liang
AU - Yeh, I. Jeng
AU - Phan, Nam Nhut
AU - Wu, Yen Hung
AU - Yen, Meng Chi
AU - Hung, Jui Hsiang
AU - Chiao, Chung Chieh
AU - Chen, Chien Fu
AU - Sun, Zhengda
AU - Jiang, Jia Zhen
AU - Hsu, Hui Ping
AU - Wang, Chih Yang
AU - Lai, Ming Derg
N1 - Funding Information:
Bioinformatics analyses and data mining were conducted at Taipei Medical University and the Bioinformatics Core at National Cheng Kung University. The authors are grateful for support from the Human Biobank, Research Center of Clinical Medicine, National Cheng Kung University Hospital. This research was funded by the Ministry of Science and Technology (MOST) of Taiwan (grants MOST105-2325-B-006-003 to M-D.L. MOST 108-2314-B-006-082 to H-P.H. MOST 108-2320-B-041 -002 to J-H.H and MOST109-2320-B-038-009-MY2 to C-Y.W.), National Cheng Kung University Hospital (grant NCKUH-10601002 to M-D.L.), Kaohsiung Medical University Hospital (KMUH108-8R72 to M-C.Y.), and Taipei Medical University (grant TMU-108-AE1-B16 to C-Y.W.). The authors give special thanks for Dr. Dan Chamberlin of his professional English editing from the office of research and development in Taipei Medical University.
Funding Information:
Bioinformatics analyses and data mining were conducted at Taipei Medical University and the Bioinformatics Core at National Cheng Kung University. The authors are grateful for support from the Human Biobank , Research Center of Clinical Medicine , National Cheng Kung University Hospital . This research was funded by the Ministry of Science and Technology (MOST) of Taiwan (grants MOST105-2325-B-006-003 to M-D.L., MOST 108-2314-B-006-082 to H-P.H., MOST 108-2320-B-041 -002 to J-H.H and MOST109-2320-B-038-009-MY2 to C-Y.W.), National Cheng Kung University Hospital (grant NCKUH-10601002 to M-D.L.), Kaohsiung Medical University Hospital ( KMUH108-8R72 to M-C.Y.), and Taipei Medical University (grant TMU-108-AE1-B16 to C-Y.W.). The authors give special thanks for Dr. Dan Chamberlin of his professional English editing from the office of research and development in Taipei Medical University.
Publisher Copyright:
© 2020 Elsevier B.V.
PY - 2020/11
Y1 - 2020/11
N2 - Coronaviruses (CoVs) consist of six strains, and the severe acute respiratory syndrome coronavirus (SARS-CoV), newly found coronavirus (SARS-CoV-2) has rapidly spread leading to a global outbreak. The ferret (Mustela putorius furo) serves as a useful animal model for studying SARS-CoV/SARS-CoV-2 infection and developing therapeutic strategies. A holistic approach for distinguishing differences in gene signatures during disease progression is lacking. The present study discovered gene expression profiles of short-term (3 days) and long-term (14 days) ferret models after SARS-CoV/SARS-CoV-2 infection using a bioinformatics approach. Through Gene Ontology (GO) and MetaCore analyses, we found that the development of stemness signaling was related to short-term SARS-CoV/SARS-CoV-2 infection. In contrast, pathways involving extracellular matrix and immune responses were associated with long-term SARS-CoV/SARS-CoV-2 infection. Some highly expressed genes in both short- and long-term models played a crucial role in the progression of SARS-CoV/SARS-CoV-2 infection, including DPP4, BMP2, NFIA, AXIN2, DAAM1, ZNF608, ME1, MGLL, LGR4, ABHD6, and ACADM. Meanwhile, we revealed that metabolic, glucocorticoid, and reactive oxygen species-associated networks were enriched in both short- and long-term infection models. The present study showed alterations in gene expressions from short-term to long-term SARS-CoV/SARS-CoV-2 infection. The current result provides an explanation of the pathophysiology for post-infectious sequelae and potential targets for treatment.
AB - Coronaviruses (CoVs) consist of six strains, and the severe acute respiratory syndrome coronavirus (SARS-CoV), newly found coronavirus (SARS-CoV-2) has rapidly spread leading to a global outbreak. The ferret (Mustela putorius furo) serves as a useful animal model for studying SARS-CoV/SARS-CoV-2 infection and developing therapeutic strategies. A holistic approach for distinguishing differences in gene signatures during disease progression is lacking. The present study discovered gene expression profiles of short-term (3 days) and long-term (14 days) ferret models after SARS-CoV/SARS-CoV-2 infection using a bioinformatics approach. Through Gene Ontology (GO) and MetaCore analyses, we found that the development of stemness signaling was related to short-term SARS-CoV/SARS-CoV-2 infection. In contrast, pathways involving extracellular matrix and immune responses were associated with long-term SARS-CoV/SARS-CoV-2 infection. Some highly expressed genes in both short- and long-term models played a crucial role in the progression of SARS-CoV/SARS-CoV-2 infection, including DPP4, BMP2, NFIA, AXIN2, DAAM1, ZNF608, ME1, MGLL, LGR4, ABHD6, and ACADM. Meanwhile, we revealed that metabolic, glucocorticoid, and reactive oxygen species-associated networks were enriched in both short- and long-term infection models. The present study showed alterations in gene expressions from short-term to long-term SARS-CoV/SARS-CoV-2 infection. The current result provides an explanation of the pathophysiology for post-infectious sequelae and potential targets for treatment.
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UR - http://www.scopus.com/inward/citedby.url?scp=85087699377&partnerID=8YFLogxK
U2 - 10.1016/j.meegid.2020.104438
DO - 10.1016/j.meegid.2020.104438
M3 - Article
C2 - 32615317
AN - SCOPUS:85087699377
SN - 1567-1348
VL - 85
JO - Infection, Genetics and Evolution
JF - Infection, Genetics and Evolution
M1 - 104438
ER -