Generation of a heterozygous p53 R249S mutant human embryonic stem cell line by TALEN-mediated genome editing

Zijun Huo, Jian Tu, An Xu, Yan Li, Donghui Wang, Mo Liu, Ruoji Zhou, Dandan Zhu, Yu Lin, Julian A. Gingold, Chia-Jui Yen, Haipeng Xiao, Ruiying Zhao

Research output: Contribution to journalArticle

Abstract

As one of the most essential genome guardians, p53 and its mutants have been suggested associated with many types of cancers. Many p53 mutants function induce unique phenotypes, including carcinogenesis, metastasis, and drug resistance. The p53(R249S) mutation is the most prevalent and specific mutation associated with liver cancer development. Here, we demonstrate the generation of a heterozygous p53(R249S) mutation in the H9 human embryonic stem cell line using TALEN-mediated genome editing. The generated cell line maintains a normal karyotype, a pluripotent state and the in vivo capacity to develop a teratoma containing all three germ layer tissues.

Original languageEnglish
Article number101360
JournalStem Cell Research
Volume34
DOIs
Publication statusPublished - 2019 Jan 1

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Cell Line
Mutation
Germ Layers
Teratoma
Liver Neoplasms
Karyotype
Drug Resistance
Carcinogenesis
Genome
Neoplasm Metastasis
Phenotype
Gene Editing
Transcription Activator-Like Effector Nucleases
Human Embryonic Stem Cells
Neoplasms

All Science Journal Classification (ASJC) codes

  • Developmental Biology
  • Cell Biology

Cite this

Huo, Zijun ; Tu, Jian ; Xu, An ; Li, Yan ; Wang, Donghui ; Liu, Mo ; Zhou, Ruoji ; Zhu, Dandan ; Lin, Yu ; Gingold, Julian A. ; Yen, Chia-Jui ; Xiao, Haipeng ; Zhao, Ruiying. / Generation of a heterozygous p53 R249S mutant human embryonic stem cell line by TALEN-mediated genome editing. In: Stem Cell Research. 2019 ; Vol. 34.
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author = "Zijun Huo and Jian Tu and An Xu and Yan Li and Donghui Wang and Mo Liu and Ruoji Zhou and Dandan Zhu and Yu Lin and Gingold, {Julian A.} and Chia-Jui Yen and Haipeng Xiao and Ruiying Zhao",
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Huo, Z, Tu, J, Xu, A, Li, Y, Wang, D, Liu, M, Zhou, R, Zhu, D, Lin, Y, Gingold, JA, Yen, C-J, Xiao, H & Zhao, R 2019, 'Generation of a heterozygous p53 R249S mutant human embryonic stem cell line by TALEN-mediated genome editing', Stem Cell Research, vol. 34, 101360. https://doi.org/10.1016/j.scr.2018.101360

Generation of a heterozygous p53 R249S mutant human embryonic stem cell line by TALEN-mediated genome editing. / Huo, Zijun; Tu, Jian; Xu, An; Li, Yan; Wang, Donghui; Liu, Mo; Zhou, Ruoji; Zhu, Dandan; Lin, Yu; Gingold, Julian A.; Yen, Chia-Jui; Xiao, Haipeng; Zhao, Ruiying.

In: Stem Cell Research, Vol. 34, 101360, 01.01.2019.

Research output: Contribution to journalArticle

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AU - Liu, Mo

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