Generation of temporal logarithmic concentration for dose-response assays on ion channels

Chang Yu Chen, Ting-Yuan Tu, De Shien Jong, Andrew M. Wo

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Citation (Scopus)

Abstract

A temporal logarithmic concentration generator in a microfluidic device is presented to integrate with planar patch-clamp chips for dose-response assays on ion channels. We propose a serial dilution principle to generate logarithmic concentrations, and the outlet concentration can be dynamically altered by different combination of valve actuations. Dose-response assays were performed on inhibitory activity of potassium channels of human embryonic kidney cells by tetraethylammonium solutions. Resulted IC50 and Hill slope reveal excellent agreement with assays from manual prepared drug concentrations showing the practicability of the present approach. It should be a great advantage for rapid drug/compound investigations for cellular studies.

Original languageEnglish
Title of host publication14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010
Pages752-754
Number of pages3
Publication statusPublished - 2010 Dec 1
Event14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010 - Groningen, Netherlands
Duration: 2010 Oct 32010 Oct 7

Publication series

Name14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010
Volume2

Other

Other14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010
CountryNetherlands
CityGroningen
Period10-10-0310-10-07

Fingerprint

Assays
Ions
Clamping devices
Microfluidics
Dilution
Potassium

All Science Journal Classification (ASJC) codes

  • Control and Systems Engineering

Cite this

Chen, C. Y., Tu, T-Y., Jong, D. S., & Wo, A. M. (2010). Generation of temporal logarithmic concentration for dose-response assays on ion channels. In 14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010 (pp. 752-754). (14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010; Vol. 2).
Chen, Chang Yu ; Tu, Ting-Yuan ; Jong, De Shien ; Wo, Andrew M. / Generation of temporal logarithmic concentration for dose-response assays on ion channels. 14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010. 2010. pp. 752-754 (14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010).
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Chen, CY, Tu, T-Y, Jong, DS & Wo, AM 2010, Generation of temporal logarithmic concentration for dose-response assays on ion channels. in 14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010. 14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010, vol. 2, pp. 752-754, 14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010, Groningen, Netherlands, 10-10-03.

Generation of temporal logarithmic concentration for dose-response assays on ion channels. / Chen, Chang Yu; Tu, Ting-Yuan; Jong, De Shien; Wo, Andrew M.

14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010. 2010. p. 752-754 (14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010; Vol. 2).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

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AU - Wo, Andrew M.

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N2 - A temporal logarithmic concentration generator in a microfluidic device is presented to integrate with planar patch-clamp chips for dose-response assays on ion channels. We propose a serial dilution principle to generate logarithmic concentrations, and the outlet concentration can be dynamically altered by different combination of valve actuations. Dose-response assays were performed on inhibitory activity of potassium channels of human embryonic kidney cells by tetraethylammonium solutions. Resulted IC50 and Hill slope reveal excellent agreement with assays from manual prepared drug concentrations showing the practicability of the present approach. It should be a great advantage for rapid drug/compound investigations for cellular studies.

AB - A temporal logarithmic concentration generator in a microfluidic device is presented to integrate with planar patch-clamp chips for dose-response assays on ion channels. We propose a serial dilution principle to generate logarithmic concentrations, and the outlet concentration can be dynamically altered by different combination of valve actuations. Dose-response assays were performed on inhibitory activity of potassium channels of human embryonic kidney cells by tetraethylammonium solutions. Resulted IC50 and Hill slope reveal excellent agreement with assays from manual prepared drug concentrations showing the practicability of the present approach. It should be a great advantage for rapid drug/compound investigations for cellular studies.

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M3 - Conference contribution

SN - 9781618390622

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Chen CY, Tu T-Y, Jong DS, Wo AM. Generation of temporal logarithmic concentration for dose-response assays on ion channels. In 14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010. 2010. p. 752-754. (14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010).