Genetic fate-mapping for studying adult cardiomyocyte replenishment after myocardial injury

Sunny S.K. Chan, Ying Zhang Shueh, Nirma Bustamante, Shih Jung Tsai, Hua Lin Wu, Jyh Hong Chen, Patrick C.H. Hsieh

Research output: Chapter in Book/Report/Conference proceedingChapter

1 Citation (Scopus)


Mounting evidence suggests the regenerative potential of the mammalian heart. Nevertheless, the contribution of endogenous stem or precursor cells to adult cardiac regeneration upon myocardial injuries remains unclear. We hereby describe a genetic fate-mapping approach to study adult cardiomyocyte replenishment after myocardial injury. Using double transgenic MerCreMer-ZEG mice, the fate of adult cardiomyocytes can be tracked by the expression of green fluorescence protein (GFP) specifically induced in cardiomyocytes. Upon experimental myocardial infarction, a reduction in GFP expression in the myocardium is observed, indicating the refreshment of cardiomyocytes by endogenous stem or precursor cells.

Original languageEnglish
Title of host publicationStem Cells for Myocardial Regeneration
Subtitle of host publicationMethods and Protocols
PublisherHumana Press Inc.
Number of pages11
ISBN (Print)9781607617044
Publication statusPublished - 2010

Publication series

NameMethods in Molecular Biology
ISSN (Print)1064-3745

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Genetics

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