Genome-wide identification of TCP genes from phalaenopsis equestris and characterization of PCF-like and CIN-like genes involved in ovule development

Yu Fu Lin, You Yi Chen, Wen Chieh Tsai

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

TEOSINTE-BRANCHED/CYCLOIDEA/PCF (TCP) proteins are plant-specific transcription factors known to have a role in multiple aspects of plant growth and development at the cellular, organ and tissue levels. There are 23 TCP genes in the genome of Phalaenopsis equestris. Phylogenetic analysis distinguished two homology classes of PeTCP transcription factor families: classes I and II. Class II was further divided into two subclasses, CIN and CYC/TB1. Spatial and temporal expression analysis indicated that PePCF10 was predominantly expressed in ovules at early developmental stages and PeCIN8 had high expression at late developmental stages in ovules, with overlapping expression at day 16 after pollination. Subcellular localization and protein-protein interaction analyses revealed that PePCF10 and PeCIN8 could form homodimers and localize in the nucleus. However, PePCF10 and PeCIN8 could not form heterodimers. In transgenic Arabidopsis thaliana plants (overexpression and SRDX, a super repression motif derived from the EAR-motif of the repression domain of tobacco ETHYLENE-RESPONSIVE ELEMENT-BINDING FACTOR 3 and SUPERMAN, dominantly repressed), the two genes could regulate cell proliferation. Together, these results suggest that PePCF10 and PeCIN8 play important roles in orchid ovule development by modulating cell division.

Original languageEnglish
Title of host publicationOrchid Biotechnology Iv
PublisherWorld Scientific Publishing Co. Pte Ltd
Pages265-288
Number of pages24
ISBN (Electronic)9789811217777
ISBN (Print)9789811217760
Publication statusPublished - 2021 Mar 23

All Science Journal Classification (ASJC) codes

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)

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