Genomic profiling of rice roots with short- and long-term chromium stress

Tsai Lien Huang, Li Yao Huang, Shih Feng Fu, Ngoc Nam Trinh, Hao-Jen Huang

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Cr(VI) is the most toxic valency form of Cr, but its toxicity targets and the cellular systems contributing to acquisition of tolerance remain to be resolved at the molecular level in plants. We used microarray assay to analyze the transcriptomic profiles of rice roots in response to Cr(VI) stress. Gene ontology analysis revealed that the 2,688 Cr-responsive genes were involved in binding activity, metabolic process, biological regulation, cellular process and catalytic activity. More transcripts were responsive to Cr(VI) during long-term exposure (24 h, 2,097 genes), than short-term exposure (1- and 3-h results pooled, 1,181 genes). Long-term Cr(VI)-regulated genes are involved in cytokinin signaling, the ubiquitin–proteasome system pathway, DNA repair and Cu transportation. The expression of AS2 transcription factors was specifically modulated by long-term Cr(VI) stress. The protein kinases receptor-like cytoplasmic kinase and receptor-like kinase in flowers 3 were significantly upregulated with only short-term Cr(VI) exposure. In addition, 4 mitogen-activated protein kinase kinase kinases, 1 mitogen-activated protein kinase (MAPK) and 1 calcium-dependent protein kinase (CDPK) were upregulated with short-term Cr(VI) treatment. Expression of reactive oxygen species and calcium and activity of MAPKs and CDPK-like kinases were induced with increasing Cr(VI) concentration. These results may provide new insights into understanding the mechanisms of Cr toxicity and tolerance during different stages in rice roots.

Original languageEnglish
Pages (from-to)157-170
Number of pages14
JournalPlant Molecular Biology
Volume86
Issue number1-2
DOIs
Publication statusPublished - 2014 Sep 1

Fingerprint

Chromium
chromium
genomics
rice
protein kinases
phosphotransferases (kinases)
genes
calcium
Phosphotransferases
mitogen-activated protein kinase kinase kinase
toxicity
receptors
Genes
chronic exposure
DNA repair
MAP Kinase Kinase Kinase 1
transcriptomics
mitogen-activated protein kinase
catalytic activity
cytokinins

All Science Journal Classification (ASJC) codes

  • Agronomy and Crop Science
  • Genetics
  • Plant Science

Cite this

Huang, Tsai Lien ; Huang, Li Yao ; Fu, Shih Feng ; Trinh, Ngoc Nam ; Huang, Hao-Jen. / Genomic profiling of rice roots with short- and long-term chromium stress. In: Plant Molecular Biology. 2014 ; Vol. 86, No. 1-2. pp. 157-170.
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Genomic profiling of rice roots with short- and long-term chromium stress. / Huang, Tsai Lien; Huang, Li Yao; Fu, Shih Feng; Trinh, Ngoc Nam; Huang, Hao-Jen.

In: Plant Molecular Biology, Vol. 86, No. 1-2, 01.09.2014, p. 157-170.

Research output: Contribution to journalArticle

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