Genomic structure, gene expression, and promoter analysis of human multidrug resistance-associated protein 7

Hsin Hsin Kao, Ming-Shi Chang, Jan Fang Cheng, Jin Ding Huang

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

The multidrug resistance-associated protein (MRP) subfamily transporters associated with anticancer drug efflux are attributed to the multidrug-resistance of cancer cells. The genomic organization of human multidrug resistance-associated protein 7 (MRP7) was identified. The human MRP7 gene, consisting of 22 exons and 21 introns, greatly differs from other members of the human MRP subfamily. A splicing variant of human MRP7, MRP7A, expressed in most human tissues, was also characterized. The 1.93-kb promoter region of MRP7 was isolated and shown to support luciferase activity at a level 4- to 5-fold greater than that of the SV40 promoter. Basal MRP7 gene expression was regulated by 2 regions in the 5′-flanking region at -1,780-1,287 bp, and at -611 to -208 bp. In Madin-Darby canine kidney (MDCK) cells, MRP7 promoter activity was increased by 226% by genotoxic 2-acetylaminofluorene and 347% by the histone deacetylase inhibitor, trichostatin A. The protein was expressed in the membrane fraction of transfected MDCK cells.

Original languageEnglish
Pages (from-to)98-110
Number of pages13
JournalJournal of biomedical science
Volume10
Issue number1
DOIs
Publication statusPublished - 2003 Feb 13

Fingerprint

Multidrug Resistance-Associated Proteins
Gene expression
Gene Expression
Madin Darby Canine Kidney Cells
trichostatin A
2-Acetylaminofluorene
Histone Deacetylase Inhibitors
5' Flanking Region
Multiple Drug Resistance
Luciferases
Genetic Promoter Regions
Introns
Exons
Genes
Cells
human ABCC10 protein
Tissue
Membranes
Pharmaceutical Preparations
Neoplasms

All Science Journal Classification (ASJC) codes

  • Endocrinology, Diabetes and Metabolism
  • Molecular Biology
  • Clinical Biochemistry
  • Cell Biology
  • Biochemistry, medical
  • Pharmacology (medical)

Cite this

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abstract = "The multidrug resistance-associated protein (MRP) subfamily transporters associated with anticancer drug efflux are attributed to the multidrug-resistance of cancer cells. The genomic organization of human multidrug resistance-associated protein 7 (MRP7) was identified. The human MRP7 gene, consisting of 22 exons and 21 introns, greatly differs from other members of the human MRP subfamily. A splicing variant of human MRP7, MRP7A, expressed in most human tissues, was also characterized. The 1.93-kb promoter region of MRP7 was isolated and shown to support luciferase activity at a level 4- to 5-fold greater than that of the SV40 promoter. Basal MRP7 gene expression was regulated by 2 regions in the 5′-flanking region at -1,780-1,287 bp, and at -611 to -208 bp. In Madin-Darby canine kidney (MDCK) cells, MRP7 promoter activity was increased by 226{\%} by genotoxic 2-acetylaminofluorene and 347{\%} by the histone deacetylase inhibitor, trichostatin A. The protein was expressed in the membrane fraction of transfected MDCK cells.",
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Genomic structure, gene expression, and promoter analysis of human multidrug resistance-associated protein 7. / Kao, Hsin Hsin; Chang, Ming-Shi; Cheng, Jan Fang; Huang, Jin Ding.

In: Journal of biomedical science, Vol. 10, No. 1, 13.02.2003, p. 98-110.

Research output: Contribution to journalArticle

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