Genotyping of hepatitis B virus - Genotypes a to G by multiplex polymerase chain reaction

Wen Chun Liu, Magnus Lindh, Maria Buti, Pham Hoang Phiet, Masashi Mizokami, Hsi Hsien Li, Koun Tem Sun, Kung Chia Young, Pin Nan Cheng, I. Chin Wu, Ting Tsung Chang

Research output: Contribution to journalArticlepeer-review

22 Citations (Scopus)


Objectives: Eight genotypes (A-H) of hepatitis B virus (HBV) are known with variations in nucleotide sequences greater than 8%. Several recent publications found that the clinical course and outcome of antiviral therapy depended on the genotype of the infecting HBV strain. Large epidemiological studies will require the availability of a system which is rapid, reliable and can be performed on a large number of samples. Methods: To establish a simple and accurate genotyping method, the study collected 369 HBV complete genomic sequences from the GenBank database. Type-specific primers were also designed that separated HBV genotypes A to G by multiplex polymerase chain reaction. Results: By comparison with the traditional restriction fragment length polymorphism method, over 93% of 441 samples were accurately genotyped by current assay, with a higher detection rate and sensitivity to detect mixed HBV infections. Conclusions: This methodology can be applied only to areas prevalent with HBV genotypes A to G. However, it provides an efficient alternative for clinical diagnosis and large-scale studies.

Original languageEnglish
Pages (from-to)247-252
Number of pages6
Issue number4
Publication statusPublished - 2008 Nov

All Science Journal Classification (ASJC) codes

  • Virology
  • Infectious Diseases


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