Glycogen synthase kinase 3 β activity is required for hBora/Aurora A-mediated mitotic entry

Yu Cheng Lee, Po Chi Liao, Yih Cherng Liou, Michael Hsiao, Chi Ying Huang, Pei Jung Lu

Research output: Contribution to journalArticlepeer-review

15 Citations (Scopus)

Abstract

The synthesis and degradation of hBora is important for the regulation of mitotic entry and exist. In G2 phase, hBora can complex with Aurora A to activate Plk1 and control mitotic entry. However, whether the post-translational modification of hBora is relevant to the mitotic entry still unclear. Here, we used the LC-MS/MS phosphopeptide mapping assay to identify 13 in vivo hBora phosphorylation sites and characterized that GSK3β can interact with hBora and phosphorylate hBora at Ser274 and Ser278. Pharmacological inhibitors of GSK3β reduced the retarded migrating band of hBora in cells and diminished the phosphorylation of hBora by in vitro kinase assay. Moreover, as well as in GSK3β activity-inhibited cells, specific knockdown of GSK3β by shRNA and S274A/S278 hBora mutant-expressing cells also exhibited the reduced Plk1 activation and a delay in mitotic entry. It suggests that GSK3β activity is required for hBora-mediated mitotic entry through Ser274 and Ser278 phosphorylation.

Original languageEnglish
Pages (from-to)953-960
Number of pages8
JournalCell Cycle
Volume12
Issue number6
DOIs
Publication statusPublished - 2013 Mar 15

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Developmental Biology
  • Cell Biology

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