Glycolytic genes are targets of the nuclear receptor Ad4BP/SF-1

Takashi Baba, Hiroyuki Otake, Tetsuya Sato, Kanako Miyabayashi, Yurina Shishido, Chia Yih Wang, Yuichi Shima, Hiroshi Kimura, Mikako Yagi, Yasuhiro Ishihara, Shinjiro Hino, Hidesato Ogawa, Mitsuyoshi Nakao, Takeshi Yamazaki, Dongchon Kang, Yasuyuki Ohkawa, Mikita Suyama, Bon Chu Chung, Ken Ichirou Morohashi

Research output: Contribution to journalArticle

35 Citations (Scopus)

Abstract

Genetic deficiencies in transcription factors can lead to the loss of certain types of cells and tissue. The steroidogenic tissue-specific nuclear receptor Ad4BP/SF-1 (NR5A1) is one such gene, because mice in which this gene is disrupted fail to develop the adrenal gland and gonads. However, the specific role of Ad4BP/SF-1 in these biological events remains unclear. Here we use chromatin immunoprecipitation sequencing to show that nearly all genes in the glycolytic pathway are regulated by Ad4BP/SF-1. Suppression of Ad4BP/SF-1 by small interfering RNA reduces production of the energy carriers ATP and nicotinamide adenine dinucleotide phosphate, as well as lowers expression of genes involved in glucose metabolism. Together, these observations may explain tissue dysgenesis as a result of Ad4BP/SF-1 gene disruption in vivo. Considering the function of estrogen-related receptor α, the present study raises the possibility that certain types of nuclear receptors regulate sets of genes involved in metabolic pathways to generate energy carriers.

Original languageEnglish
Article number3634
JournalNature communications
Volume5
DOIs
Publication statusPublished - 2014 Apr 14

Fingerprint

Cytoplasmic and Nuclear Receptors
genes
Genes
Tissue
adrenal gland
gonads
Chromatin Immunoprecipitation
estrogens
Gonads
nicotinamide
Adrenal Glands
chromatin
Metabolic Networks and Pathways
NADP
sequencing
adenosine triphosphate
Estrogen Receptors
adenines
Small Interfering RNA
metabolism

All Science Journal Classification (ASJC) codes

  • Chemistry(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Physics and Astronomy(all)

Cite this

Baba, T., Otake, H., Sato, T., Miyabayashi, K., Shishido, Y., Wang, C. Y., ... Morohashi, K. I. (2014). Glycolytic genes are targets of the nuclear receptor Ad4BP/SF-1. Nature communications, 5, [3634]. https://doi.org/10.1038/ncomms4634
Baba, Takashi ; Otake, Hiroyuki ; Sato, Tetsuya ; Miyabayashi, Kanako ; Shishido, Yurina ; Wang, Chia Yih ; Shima, Yuichi ; Kimura, Hiroshi ; Yagi, Mikako ; Ishihara, Yasuhiro ; Hino, Shinjiro ; Ogawa, Hidesato ; Nakao, Mitsuyoshi ; Yamazaki, Takeshi ; Kang, Dongchon ; Ohkawa, Yasuyuki ; Suyama, Mikita ; Chung, Bon Chu ; Morohashi, Ken Ichirou. / Glycolytic genes are targets of the nuclear receptor Ad4BP/SF-1. In: Nature communications. 2014 ; Vol. 5.
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Baba, T, Otake, H, Sato, T, Miyabayashi, K, Shishido, Y, Wang, CY, Shima, Y, Kimura, H, Yagi, M, Ishihara, Y, Hino, S, Ogawa, H, Nakao, M, Yamazaki, T, Kang, D, Ohkawa, Y, Suyama, M, Chung, BC & Morohashi, KI 2014, 'Glycolytic genes are targets of the nuclear receptor Ad4BP/SF-1', Nature communications, vol. 5, 3634. https://doi.org/10.1038/ncomms4634

Glycolytic genes are targets of the nuclear receptor Ad4BP/SF-1. / Baba, Takashi; Otake, Hiroyuki; Sato, Tetsuya; Miyabayashi, Kanako; Shishido, Yurina; Wang, Chia Yih; Shima, Yuichi; Kimura, Hiroshi; Yagi, Mikako; Ishihara, Yasuhiro; Hino, Shinjiro; Ogawa, Hidesato; Nakao, Mitsuyoshi; Yamazaki, Takeshi; Kang, Dongchon; Ohkawa, Yasuyuki; Suyama, Mikita; Chung, Bon Chu; Morohashi, Ken Ichirou.

In: Nature communications, Vol. 5, 3634, 14.04.2014.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Glycolytic genes are targets of the nuclear receptor Ad4BP/SF-1

AU - Baba, Takashi

AU - Otake, Hiroyuki

AU - Sato, Tetsuya

AU - Miyabayashi, Kanako

AU - Shishido, Yurina

AU - Wang, Chia Yih

AU - Shima, Yuichi

AU - Kimura, Hiroshi

AU - Yagi, Mikako

AU - Ishihara, Yasuhiro

AU - Hino, Shinjiro

AU - Ogawa, Hidesato

AU - Nakao, Mitsuyoshi

AU - Yamazaki, Takeshi

AU - Kang, Dongchon

AU - Ohkawa, Yasuyuki

AU - Suyama, Mikita

AU - Chung, Bon Chu

AU - Morohashi, Ken Ichirou

PY - 2014/4/14

Y1 - 2014/4/14

N2 - Genetic deficiencies in transcription factors can lead to the loss of certain types of cells and tissue. The steroidogenic tissue-specific nuclear receptor Ad4BP/SF-1 (NR5A1) is one such gene, because mice in which this gene is disrupted fail to develop the adrenal gland and gonads. However, the specific role of Ad4BP/SF-1 in these biological events remains unclear. Here we use chromatin immunoprecipitation sequencing to show that nearly all genes in the glycolytic pathway are regulated by Ad4BP/SF-1. Suppression of Ad4BP/SF-1 by small interfering RNA reduces production of the energy carriers ATP and nicotinamide adenine dinucleotide phosphate, as well as lowers expression of genes involved in glucose metabolism. Together, these observations may explain tissue dysgenesis as a result of Ad4BP/SF-1 gene disruption in vivo. Considering the function of estrogen-related receptor α, the present study raises the possibility that certain types of nuclear receptors regulate sets of genes involved in metabolic pathways to generate energy carriers.

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