Glyoxalase-I is a novel prognosis factor associated with gastric cancer progression

Wan Li Cheng, Ming Ming Tsai, Chung Ying Tsai, Ya Hui Huang, Cheng Yi Chen, Hsiang Cheng Chi, Yi Hsin Tseng, Im Wai Chao, Wei Chi Lin, Sheng Ming Wu, Ying Liang, Chia Jung Liao, Yang Hsiang Lin, I. Hsiao Chung, Wei Jan Chen, Paul Y. Lin, Chia Siu Wang, Kwang Huei Lin

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

Glyoxalase I (GLO1), a methylglyoxal detoxification enzyme, is implicated in the progression of human malignancies. The role of GLO1 in gastric cancer development or progression is currently unclear. The expression of GLO1 was determined in primary gastric cancer specimens using quantitative polymerase chain reaction, immunohistochemistry (IHC), and western blotting analyses. GLO1 expression was higher in gastric cancer tissues, compared with that in adjacent noncancerous tissues. Elevated expression of GLO1 was significantly associated with gastric wall invasion, lymph node metastasis, and pathological stage, suggesting a novel role of GLO1 in gastric cancer development and progression. The 5-year survival rate of the lower GLO1 expression groups was significantly greater than that of the higher expression groups (log rank P = 0.0373) in IHC experiments. Over-expression of GLO1 in gastric cancer cell lines increases cell proliferation, migration and invasiveness. Conversely, down-regulation of GLO1 with shRNA led to a marked reduction in the migration and invasion abilities. Our data strongly suggest that high expression of GLO1 in gastric cancer enhances the metastasis ability of tumor cells in vitro and in vivo, and support its efficacy as a potential marker for the detection and prognosis of gastric cancer.

Original languageEnglish
Article numbere34352
JournalPloS one
Volume7
Issue number3
DOIs
Publication statusPublished - 2012 Mar 29

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)
  • General

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