Gravity-driven microhydrodynamics-based cell sorter (microHYCS) for rapid, inexpensive, and efficient cell separation and size-profiling

Dongeun Huh, Hsien-Hung Wei, O. D. Kripfgans, J. B. Fowlkes, J. B. Grotberg, S. Takayama

Research output: Chapter in Book/Report/Conference proceedingConference contribution

9 Citations (Scopus)

Abstract

Describes a new type of gravity-driven polymeric microfluidic cell sorter developed for low-power, low-cost, and rapid cell separation. The device takes advantage of size-based differential migration of cells/particles in flows where widening of flow streamlines amplifies the separation between sample particles. We studied the migration and separation of polystyrene microbeads, red blood cells, and perfluorocarbon droplets used for ultrasound imaging and therapy having different sizes in the novel cell sorter. Compared to other conventional cell separation systems, microHYCS reduces power and cost requirements, and is advantageous for the development of easy-to-use sample separation and diagnostics tools.

Original languageEnglish
Title of host publication2nd Annual International IEEE-EMBS Special Topic Conference on Microtechnologies in Medicine and Biology - Proceedings
EditorsDavid Beebe, Andre Dittmar
PublisherInstitute of Electrical and Electronics Engineers Inc.
Pages466-469
Number of pages4
ISBN (Electronic)0780374800, 9780780374805
DOIs
Publication statusPublished - 2002 Jan 1
Event2nd Annual International IEEE-EMBS Special Topic Conference on Microtechnologies in Medicine and Biology - Madison, United States
Duration: 2002 May 22002 May 4

Publication series

Name2nd Annual International IEEE-EMBS Special Topic Conference on Microtechnologies in Medicine and Biology - Proceedings

Other

Other2nd Annual International IEEE-EMBS Special Topic Conference on Microtechnologies in Medicine and Biology
CountryUnited States
CityMadison
Period02-05-0202-05-04

Fingerprint

Cell Separation
Gravitation
Cell Size
Fluorocarbons
Costs and Cost Analysis
Microfluidics
Polystyrenes
Microspheres
Cell Movement
Ultrasonography
Erythrocytes
Equipment and Supplies
Therapeutics

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Microbiology (medical)

Cite this

Huh, D., Wei, H-H., Kripfgans, O. D., Fowlkes, J. B., Grotberg, J. B., & Takayama, S. (2002). Gravity-driven microhydrodynamics-based cell sorter (microHYCS) for rapid, inexpensive, and efficient cell separation and size-profiling. In D. Beebe, & A. Dittmar (Eds.), 2nd Annual International IEEE-EMBS Special Topic Conference on Microtechnologies in Medicine and Biology - Proceedings (pp. 466-469). [1002375] (2nd Annual International IEEE-EMBS Special Topic Conference on Microtechnologies in Medicine and Biology - Proceedings). Institute of Electrical and Electronics Engineers Inc.. https://doi.org/10.1109/MMB.2002.1002375
Huh, Dongeun ; Wei, Hsien-Hung ; Kripfgans, O. D. ; Fowlkes, J. B. ; Grotberg, J. B. ; Takayama, S. / Gravity-driven microhydrodynamics-based cell sorter (microHYCS) for rapid, inexpensive, and efficient cell separation and size-profiling. 2nd Annual International IEEE-EMBS Special Topic Conference on Microtechnologies in Medicine and Biology - Proceedings. editor / David Beebe ; Andre Dittmar. Institute of Electrical and Electronics Engineers Inc., 2002. pp. 466-469 (2nd Annual International IEEE-EMBS Special Topic Conference on Microtechnologies in Medicine and Biology - Proceedings).
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Huh, D, Wei, H-H, Kripfgans, OD, Fowlkes, JB, Grotberg, JB & Takayama, S 2002, Gravity-driven microhydrodynamics-based cell sorter (microHYCS) for rapid, inexpensive, and efficient cell separation and size-profiling. in D Beebe & A Dittmar (eds), 2nd Annual International IEEE-EMBS Special Topic Conference on Microtechnologies in Medicine and Biology - Proceedings., 1002375, 2nd Annual International IEEE-EMBS Special Topic Conference on Microtechnologies in Medicine and Biology - Proceedings, Institute of Electrical and Electronics Engineers Inc., pp. 466-469, 2nd Annual International IEEE-EMBS Special Topic Conference on Microtechnologies in Medicine and Biology, Madison, United States, 02-05-02. https://doi.org/10.1109/MMB.2002.1002375

Gravity-driven microhydrodynamics-based cell sorter (microHYCS) for rapid, inexpensive, and efficient cell separation and size-profiling. / Huh, Dongeun; Wei, Hsien-Hung; Kripfgans, O. D.; Fowlkes, J. B.; Grotberg, J. B.; Takayama, S.

2nd Annual International IEEE-EMBS Special Topic Conference on Microtechnologies in Medicine and Biology - Proceedings. ed. / David Beebe; Andre Dittmar. Institute of Electrical and Electronics Engineers Inc., 2002. p. 466-469 1002375 (2nd Annual International IEEE-EMBS Special Topic Conference on Microtechnologies in Medicine and Biology - Proceedings).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

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AB - Describes a new type of gravity-driven polymeric microfluidic cell sorter developed for low-power, low-cost, and rapid cell separation. The device takes advantage of size-based differential migration of cells/particles in flows where widening of flow streamlines amplifies the separation between sample particles. We studied the migration and separation of polystyrene microbeads, red blood cells, and perfluorocarbon droplets used for ultrasound imaging and therapy having different sizes in the novel cell sorter. Compared to other conventional cell separation systems, microHYCS reduces power and cost requirements, and is advantageous for the development of easy-to-use sample separation and diagnostics tools.

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Huh D, Wei H-H, Kripfgans OD, Fowlkes JB, Grotberg JB, Takayama S. Gravity-driven microhydrodynamics-based cell sorter (microHYCS) for rapid, inexpensive, and efficient cell separation and size-profiling. In Beebe D, Dittmar A, editors, 2nd Annual International IEEE-EMBS Special Topic Conference on Microtechnologies in Medicine and Biology - Proceedings. Institute of Electrical and Electronics Engineers Inc. 2002. p. 466-469. 1002375. (2nd Annual International IEEE-EMBS Special Topic Conference on Microtechnologies in Medicine and Biology - Proceedings). https://doi.org/10.1109/MMB.2002.1002375