Green synthesis of gold nanoparticle/gelatin/protein nanogels with enhanced bioluminescence/biofluorescence

I. Hsiu Chen, Yu Fon Chen, Jhih Han Liou, Jinn Tsyy Lai, Chia Chen Hsu, Nai Yi Wang, Jeng Shiung Jan

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Here we report the green synthesis of gelatin/protein hybrid nanogels containing gold nanoparticles (AuNPs) that collectively exhibit metal-enhanced luminescence/fluorescence (MEL/MEF). The gelatin/protein nanogels, prepared by genipin cross-linking of preformed gelatin/protein polyion complexes (PICs), exhibited sizes ranging between 50 and 200 nm, depending on the weight ratio of gelatin and protein. These nanogels serve as reducing and stabilizing agents for the AuNPs, allowing for nucleation in a gel network that exhibits colloidal stability and MEL/MEF. AuNP/gelatin/HRP and AuNP/gelatin/LTF nanogels presented an ~11-fold enhancement of bioluminescence in an HRP-luminol system and a ~50-fold fluorescence enhancement when compared to free LTF in cell uptake experiments. These hybrid nanogels show promise for optically enhanced diagnosis and other therapeutic applications.

Original languageEnglish
Article number110101
JournalMaterials Science and Engineering C
Volume105
DOIs
Publication statusPublished - 2019 Dec

Fingerprint

Bioluminescence
bioluminescence
gelatins
Gelatin
Gold
gold
Nanoparticles
proteins
Proteins
nanoparticles
Fluorescence
synthesis
Luminescence
fluorescence
Metals
luminescence
Luminol
Nucleation
Gels
augmentation

All Science Journal Classification (ASJC) codes

  • Materials Science(all)
  • Condensed Matter Physics
  • Mechanics of Materials
  • Mechanical Engineering

Cite this

Chen, I. Hsiu ; Chen, Yu Fon ; Liou, Jhih Han ; Lai, Jinn Tsyy ; Hsu, Chia Chen ; Wang, Nai Yi ; Jan, Jeng Shiung. / Green synthesis of gold nanoparticle/gelatin/protein nanogels with enhanced bioluminescence/biofluorescence. In: Materials Science and Engineering C. 2019 ; Vol. 105.
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abstract = "Here we report the green synthesis of gelatin/protein hybrid nanogels containing gold nanoparticles (AuNPs) that collectively exhibit metal-enhanced luminescence/fluorescence (MEL/MEF). The gelatin/protein nanogels, prepared by genipin cross-linking of preformed gelatin/protein polyion complexes (PICs), exhibited sizes ranging between 50 and 200 nm, depending on the weight ratio of gelatin and protein. These nanogels serve as reducing and stabilizing agents for the AuNPs, allowing for nucleation in a gel network that exhibits colloidal stability and MEL/MEF. AuNP/gelatin/HRP and AuNP/gelatin/LTF nanogels presented an ~11-fold enhancement of bioluminescence in an HRP-luminol system and a ~50-fold fluorescence enhancement when compared to free LTF in cell uptake experiments. These hybrid nanogels show promise for optically enhanced diagnosis and other therapeutic applications.",
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Green synthesis of gold nanoparticle/gelatin/protein nanogels with enhanced bioluminescence/biofluorescence. / Chen, I. Hsiu; Chen, Yu Fon; Liou, Jhih Han; Lai, Jinn Tsyy; Hsu, Chia Chen; Wang, Nai Yi; Jan, Jeng Shiung.

In: Materials Science and Engineering C, Vol. 105, 110101, 12.2019.

Research output: Contribution to journalArticle

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