Green synthesis of gold nanoparticle/gelatin/protein nanogels with enhanced bioluminescence/biofluorescence

I. Hsiu Chen, Yu Fon Chen, Jhih Han Liou, Jinn Tsyy Lai, Chia Chen Hsu, Nai Yi Wang, Jeng Shiung Jan

Research output: Contribution to journalArticlepeer-review

7 Citations (Scopus)


Here we report the green synthesis of gelatin/protein hybrid nanogels containing gold nanoparticles (AuNPs) that collectively exhibit metal-enhanced luminescence/fluorescence (MEL/MEF). The gelatin/protein nanogels, prepared by genipin cross-linking of preformed gelatin/protein polyion complexes (PICs), exhibited sizes ranging between 50 and 200 nm, depending on the weight ratio of gelatin and protein. These nanogels serve as reducing and stabilizing agents for the AuNPs, allowing for nucleation in a gel network that exhibits colloidal stability and MEL/MEF. AuNP/gelatin/HRP and AuNP/gelatin/LTF nanogels presented an ~11-fold enhancement of bioluminescence in an HRP-luminol system and a ~50-fold fluorescence enhancement when compared to free LTF in cell uptake experiments. These hybrid nanogels show promise for optically enhanced diagnosis and other therapeutic applications.

Original languageEnglish
Article number110101
JournalMaterials Science and Engineering C
Publication statusPublished - 2019 Dec

All Science Journal Classification (ASJC) codes

  • Materials Science(all)
  • Condensed Matter Physics
  • Mechanics of Materials
  • Mechanical Engineering

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