Gypenosides causes DNA damage and inhibits expression of DNA repair genes of human oral cancer SAS cells

Kung Wen Lu, Jung Chou Chen, Tung Yuan Lai, Jai Sing Yang, Shu Wen Weng, Yi Shih Ma, Nou Ying Tang, Pei-Jung Lu, Jing Ru Weng, Jing Gung Chung

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Gypenosides (Gyp) are the major components of Gynostemma pentaphyllum Makino, a Chinese medical plant. Recently, Gyp has been shown to induce cell cycle arrest and apoptosis in many human cancer cell lines. However, there is no available information to address the effects of Gyp on DNA damage and DNA repair-associated gene expression in human oral cancer cells. Therefore, we investigated whether Gyp induced DNA damage and DNA repair gene expression in human oral cancer SAS cells. The results from flow cytometric assay indicated that Gyp-induced cytotoxic effects led to a decrease in the percentage of viable SAS cells. The results from comet assay revealed that the incubation of SAS cells with Gyp led to a longer DNA migration smear (comet tail) when compared with control and this effect was dose-dependent. The results from real-time PCR analysis indicated that treatment of SAS cells with 180 μg/ml of Gyp for 24 h led to a decrease in 14-3-3σ, DNA-dependent serine/threonine protein kinase (DNAPK), p53, ataxia telangiectasia mutated (ATM), ataxia-telangiectasia and Rad3-related (ATR) and breast cancer gene 1 (BRCA1) mRNA expression. These observations may explain the cell death caused by Gyp in SAS cells. Taken together, Gyp induced DNA damage and inhibited DNA repair-associated gene expressions in human oral cancer SAS cells in vitro.

Original languageEnglish
Pages (from-to)287-291
Number of pages5
JournalIn Vivo
Volume24
Issue number3
Publication statusPublished - 2010 May

Fingerprint

Mouth Neoplasms
DNA Repair
DNA Damage
Repair
Genes
DNA
Gene expression
Ataxia Telangiectasia
Cells
Gene Expression
Gynostemma
Assays
DNA-Activated Protein Kinase
gypenoside
Comet Assay
Neoplasm Genes
Cell death
Cell Cycle Checkpoints
Tail
Real-Time Polymerase Chain Reaction

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)
  • Pharmacology

Cite this

Lu, K. W., Chen, J. C., Lai, T. Y., Yang, J. S., Weng, S. W., Ma, Y. S., ... Chung, J. G. (2010). Gypenosides causes DNA damage and inhibits expression of DNA repair genes of human oral cancer SAS cells. In Vivo, 24(3), 287-291.
Lu, Kung Wen ; Chen, Jung Chou ; Lai, Tung Yuan ; Yang, Jai Sing ; Weng, Shu Wen ; Ma, Yi Shih ; Tang, Nou Ying ; Lu, Pei-Jung ; Weng, Jing Ru ; Chung, Jing Gung. / Gypenosides causes DNA damage and inhibits expression of DNA repair genes of human oral cancer SAS cells. In: In Vivo. 2010 ; Vol. 24, No. 3. pp. 287-291.
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abstract = "Gypenosides (Gyp) are the major components of Gynostemma pentaphyllum Makino, a Chinese medical plant. Recently, Gyp has been shown to induce cell cycle arrest and apoptosis in many human cancer cell lines. However, there is no available information to address the effects of Gyp on DNA damage and DNA repair-associated gene expression in human oral cancer cells. Therefore, we investigated whether Gyp induced DNA damage and DNA repair gene expression in human oral cancer SAS cells. The results from flow cytometric assay indicated that Gyp-induced cytotoxic effects led to a decrease in the percentage of viable SAS cells. The results from comet assay revealed that the incubation of SAS cells with Gyp led to a longer DNA migration smear (comet tail) when compared with control and this effect was dose-dependent. The results from real-time PCR analysis indicated that treatment of SAS cells with 180 μg/ml of Gyp for 24 h led to a decrease in 14-3-3σ, DNA-dependent serine/threonine protein kinase (DNAPK), p53, ataxia telangiectasia mutated (ATM), ataxia-telangiectasia and Rad3-related (ATR) and breast cancer gene 1 (BRCA1) mRNA expression. These observations may explain the cell death caused by Gyp in SAS cells. Taken together, Gyp induced DNA damage and inhibited DNA repair-associated gene expressions in human oral cancer SAS cells in vitro.",
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Lu, KW, Chen, JC, Lai, TY, Yang, JS, Weng, SW, Ma, YS, Tang, NY, Lu, P-J, Weng, JR & Chung, JG 2010, 'Gypenosides causes DNA damage and inhibits expression of DNA repair genes of human oral cancer SAS cells', In Vivo, vol. 24, no. 3, pp. 287-291.

Gypenosides causes DNA damage and inhibits expression of DNA repair genes of human oral cancer SAS cells. / Lu, Kung Wen; Chen, Jung Chou; Lai, Tung Yuan; Yang, Jai Sing; Weng, Shu Wen; Ma, Yi Shih; Tang, Nou Ying; Lu, Pei-Jung; Weng, Jing Ru; Chung, Jing Gung.

In: In Vivo, Vol. 24, No. 3, 05.2010, p. 287-291.

Research output: Contribution to journalArticle

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AU - Lu, Kung Wen

AU - Chen, Jung Chou

AU - Lai, Tung Yuan

AU - Yang, Jai Sing

AU - Weng, Shu Wen

AU - Ma, Yi Shih

AU - Tang, Nou Ying

AU - Lu, Pei-Jung

AU - Weng, Jing Ru

AU - Chung, Jing Gung

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Lu KW, Chen JC, Lai TY, Yang JS, Weng SW, Ma YS et al. Gypenosides causes DNA damage and inhibits expression of DNA repair genes of human oral cancer SAS cells. In Vivo. 2010 May;24(3):287-291.