Gypenosides causes DNA damage and inhibits expression of DNA repair genes of human oral cancer SAS cells

Kung Wen Lu, Jung Chou Chen, Tung Yuan Lai, Jai Sing Yang, Shu Wen Weng, Yi Shih Ma, Nou Ying Tang, Pei Jung Lu, Jing Ru Weng, Jing Gung Chung

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Gypenosides (Gyp) are the major components of Gynostemma pentaphyllum Makino, a Chinese medical plant. Recently, Gyp has been shown to induce cell cycle arrest and apoptosis in many human cancer cell lines. However, there is no available information to address the effects of Gyp on DNA damage and DNA repair-associated gene expression in human oral cancer cells. Therefore, we investigated whether Gyp induced DNA damage and DNA repair gene expression in human oral cancer SAS cells. The results from flow cytometric assay indicated that Gyp-induced cytotoxic effects led to a decrease in the percentage of viable SAS cells. The results from comet assay revealed that the incubation of SAS cells with Gyp led to a longer DNA migration smear (comet tail) when compared with control and this effect was dose-dependent. The results from real-time PCR analysis indicated that treatment of SAS cells with 180 μg/ml of Gyp for 24 h led to a decrease in 14-3-3σ, DNA-dependent serine/threonine protein kinase (DNAPK), p53, ataxia telangiectasia mutated (ATM), ataxia-telangiectasia and Rad3-related (ATR) and breast cancer gene 1 (BRCA1) mRNA expression. These observations may explain the cell death caused by Gyp in SAS cells. Taken together, Gyp induced DNA damage and inhibited DNA repair-associated gene expressions in human oral cancer SAS cells in vitro.

Original languageEnglish
Pages (from-to)287-291
Number of pages5
JournalIn Vivo
Volume24
Issue number3
Publication statusPublished - 2010 May 1

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)
  • Pharmacology

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