Haloterrigena mahii sp. nov., an extremely halophilic archaeon from a solar saltern

Jiun Yan Ding, Sheng Chung Chen, Mei Chin Lai, Tsai Ling Liao

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

A pleomorphic, gas-vesicle-containing, halophilic archaeon, designated strain H13T, was isolated from the solar saltern of the Western Salt Co., Chula Vista, California, USA. Cells of strain H13T were non-motile, rod-shaped and 3–10μm in length. The optimum growth conditions were 3.5–5.0 M NaCl, 45–55 °C, and pH range of 6.5–8.2. The major polar lipids were C20C20 and C20C25 derivatives of phosphatidylglycerol, phosphatidylglycerol phosphate methyl ester and disulfated diglycosyl diether-1. The G+C content of he genome of strain H13T was calculated as 65.10 mol%. Phylogenetic analysis of 16S rRNA and rpoB′ genes revealed that strain H13 was most closely related to Haloterrigena saccharevitans AB14T (16S rRNA gene sequence similarity: 99.51%; rpoB′ sequence similarity: 96.19%) and Haloterrigena thermotolerans PR5T (99.11%; 95.50%). Strain H13T showed low genome relatedness values with Htg. saccharevitans AB14T and Htg. thermotolerans PR5T based on estimated average nucleotide identity (ANI; 92.59 and 91.68%, respectively) and genome-to-genome distance analysis (GGDA; 47.90 and 45.00%, respectively). Based on the phenotypic, chemotaxonomic and phylogenetic properties and the genome relatedness, it is evident that strain H13T represents a novel species of the genus Haloterrigena, for which the name Haloterrigena mahiisp. nov. is proposed. The type strain is H13T (=BCRC 910151T=NBRC 111885T).

Original languageEnglish
Article number001811
Pages (from-to)1333-1338
Number of pages6
JournalInternational Journal of Systematic and Evolutionary Microbiology
Volume67
Issue number5
DOIs
Publication statusPublished - 2017 May

Fingerprint

Archaea
genome
Genome
new species
rRNA Genes
relatedness
phosphatidylglycerols
phylogenetics
Phosphatidylglycerols
gene
Base Composition
ribosomal RNA
vesicle
ester
Names
Esters
phylogeny
Nucleotides
Salts
Gases

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Ecology, Evolution, Behavior and Systematics

Cite this

@article{852682ffaf0c483d8bd9d073c4c038f0,
title = "Haloterrigena mahii sp. nov., an extremely halophilic archaeon from a solar saltern",
abstract = "A pleomorphic, gas-vesicle-containing, halophilic archaeon, designated strain H13T, was isolated from the solar saltern of the Western Salt Co., Chula Vista, California, USA. Cells of strain H13T were non-motile, rod-shaped and 3–10μm in length. The optimum growth conditions were 3.5–5.0 M NaCl, 45–55 °C, and pH range of 6.5–8.2. The major polar lipids were C20C20 and C20C25 derivatives of phosphatidylglycerol, phosphatidylglycerol phosphate methyl ester and disulfated diglycosyl diether-1. The G+C content of he genome of strain H13T was calculated as 65.10 mol{\%}. Phylogenetic analysis of 16S rRNA and rpoB′ genes revealed that strain H13 was most closely related to Haloterrigena saccharevitans AB14T (16S rRNA gene sequence similarity: 99.51{\%}; rpoB′ sequence similarity: 96.19{\%}) and Haloterrigena thermotolerans PR5T (99.11{\%}; 95.50{\%}). Strain H13T showed low genome relatedness values with Htg. saccharevitans AB14T and Htg. thermotolerans PR5T based on estimated average nucleotide identity (ANI; 92.59 and 91.68{\%}, respectively) and genome-to-genome distance analysis (GGDA; 47.90 and 45.00{\%}, respectively). Based on the phenotypic, chemotaxonomic and phylogenetic properties and the genome relatedness, it is evident that strain H13T represents a novel species of the genus Haloterrigena, for which the name Haloterrigena mahiisp. nov. is proposed. The type strain is H13T (=BCRC 910151T=NBRC 111885T).",
author = "Ding, {Jiun Yan} and Chen, {Sheng Chung} and Lai, {Mei Chin} and Liao, {Tsai Ling}",
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Haloterrigena mahii sp. nov., an extremely halophilic archaeon from a solar saltern. / Ding, Jiun Yan; Chen, Sheng Chung; Lai, Mei Chin; Liao, Tsai Ling.

In: International Journal of Systematic and Evolutionary Microbiology, Vol. 67, No. 5, 001811, 05.2017, p. 1333-1338.

Research output: Contribution to journalArticle

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AB - A pleomorphic, gas-vesicle-containing, halophilic archaeon, designated strain H13T, was isolated from the solar saltern of the Western Salt Co., Chula Vista, California, USA. Cells of strain H13T were non-motile, rod-shaped and 3–10μm in length. The optimum growth conditions were 3.5–5.0 M NaCl, 45–55 °C, and pH range of 6.5–8.2. The major polar lipids were C20C20 and C20C25 derivatives of phosphatidylglycerol, phosphatidylglycerol phosphate methyl ester and disulfated diglycosyl diether-1. The G+C content of he genome of strain H13T was calculated as 65.10 mol%. Phylogenetic analysis of 16S rRNA and rpoB′ genes revealed that strain H13 was most closely related to Haloterrigena saccharevitans AB14T (16S rRNA gene sequence similarity: 99.51%; rpoB′ sequence similarity: 96.19%) and Haloterrigena thermotolerans PR5T (99.11%; 95.50%). Strain H13T showed low genome relatedness values with Htg. saccharevitans AB14T and Htg. thermotolerans PR5T based on estimated average nucleotide identity (ANI; 92.59 and 91.68%, respectively) and genome-to-genome distance analysis (GGDA; 47.90 and 45.00%, respectively). Based on the phenotypic, chemotaxonomic and phylogenetic properties and the genome relatedness, it is evident that strain H13T represents a novel species of the genus Haloterrigena, for which the name Haloterrigena mahiisp. nov. is proposed. The type strain is H13T (=BCRC 910151T=NBRC 111885T).

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