HbiF regulates type 1 fimbriation independently of FimB and FimE

Yi Xie, Yufeng Yao, Vitaliy Kolisnychenko, Ching Hao Teng, Kwang Sik Kim

Research output: Contribution to journalArticle

35 Citations (Scopus)

Abstract

Type 1 fimbriae have been suggested to play a role in the pathogenesis of extraintestinal Escherichia coli infection. Type 1 fimbriation in E. coli is phase variable and known to be dependent upon FimB and FimE, the two recombinases that invert the molecular switch fimS and control the expression of the downstream fim operon. Here we showed that HbiF, a novel site-specific recombinase, inverted fimS independently of FimB and FimE. HbiF-mediated fimS inversion appeared to be predominantly switching from "off" (termed OFF) to "on" (termed ON) orientation. This is different from the fimS inversion mediated by either FimB (bidirectional ON to OFF and OFF to ON) or FimE (unidirectional ON to OFF). Constitutive expression of the hbiF gene in E. coli resulted in a fimS-locked-ON phenotype, which resulted in the pathogenic E. coli K1 strain being incapable of inducing a high degree of bacteremia in neonatal rats. Discovery of HbiF-mediated OFF-to-ON fimS switching provides a new opportunity to develop a strategy for the prevention and therapy of extraintestinal E. coli infection including bacteremia and meningitis.

Original languageEnglish
Pages (from-to)4039-4047
Number of pages9
JournalInfection and Immunity
Volume74
Issue number7
DOIs
Publication statusPublished - 2006 Jul 1

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Escherichia coli Infections
Escherichia coli
Bacteremia
Recombinases
Operon
Meningitis
Phenotype
Gene Expression
Therapeutics
Site-specific recombinase

All Science Journal Classification (ASJC) codes

  • Parasitology
  • Microbiology
  • Immunology
  • Infectious Diseases

Cite this

Xie, Yi ; Yao, Yufeng ; Kolisnychenko, Vitaliy ; Teng, Ching Hao ; Kim, Kwang Sik. / HbiF regulates type 1 fimbriation independently of FimB and FimE. In: Infection and Immunity. 2006 ; Vol. 74, No. 7. pp. 4039-4047.
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HbiF regulates type 1 fimbriation independently of FimB and FimE. / Xie, Yi; Yao, Yufeng; Kolisnychenko, Vitaliy; Teng, Ching Hao; Kim, Kwang Sik.

In: Infection and Immunity, Vol. 74, No. 7, 01.07.2006, p. 4039-4047.

Research output: Contribution to journalArticle

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