Hemodynamic flow visualization of early embryonic great vessels using μPIV

Selda Goktas, Chia Yuan Chen, William J. Kowalski, Kerem Pekkan

Research output: Contribution to journalArticlepeer-review

3 Citations (Scopus)

Abstract

Microparticle image velocimetry (μPIV) is an evolving quantitative methodology to closely and accurately monitor the cardiac flow dynamics and mechanotransduction during vascular morphogenesis. While PIV technique has a long history, contemporary developments in advanced microscopy have significantly expanded its power. This chapter includes three new methods for μPIV acquisition in selected embryonic structures achieved through advanced optical imaging: (1) high-speed confocal scanning of transgenic zebrafish embryos, where the transgenic erythrocytes act as the tracing particles; (2) microinjection of artificial seeding particles in chick embryos visualized with stereomicroscopy; and (3) real-time, timeresolved optical coherence tomography acquisition of vitelline vessel flow profiles in chick embryos, tracking the erythrocytes.

Original languageEnglish
Pages (from-to)17-30
Number of pages14
JournalMethods in Molecular Biology
Volume1189
DOIs
Publication statusPublished - 2015 Jan 1

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Genetics

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