Heterologous protection in pigs induced by a plasmid-cured and crp gene-deleted Salmonella choleraesuis live vaccine

Chun Yen Chu, Shiang Yiu Wang, Zeng Weng Chen, Maw Sheng Chien, Ji Ping Huang, Jen Jin Chen, Li Shian Hong, Ai Li Shiau, Jeng Liang Tsai, Chao Liang Wu

Research output: Contribution to journalArticlepeer-review

11 Citations (Scopus)

Abstract

In this study, we exploited a crp (cAMP receptor protein) gene-deleted, virulence plasmid-cured Salmonella choleraesuis mutant with decreased carbon source utilization, designated S.C.-Δcrp/vpl-, as a live vaccine strain. Normal weight gain with no clinical signs was observed in pigs immunized with high doses of S.C.-Δcrp/vpl- live vaccine. Vaccination in pregnant sows induced high maternal antibodies, which could prevent piglets from Salmonella infection. Moreover, serial transmission of the vaccine strain in piglets produced no evidence of reversion to virulence. Furthermore, the peripheral blood mononuclear cells from immunized piglets also developed Salmonella specific T-cell proliferative response in vitro. Our results indicate that immunogenic antigens in S.C.-Δcrp/vpl- can induce adequate immunity to protect pigs against challenge with a heterologous virulent strain. Thus, this mutant holds promise for the development of a new live S. choleraesuis vaccine.

Original languageEnglish
Pages (from-to)7031-7040
Number of pages10
JournalVaccine
Volume25
Issue number41
DOIs
Publication statusPublished - 2007 Oct 10

All Science Journal Classification (ASJC) codes

  • Molecular Medicine
  • Immunology and Microbiology(all)
  • veterinary(all)
  • Public Health, Environmental and Occupational Health
  • Infectious Diseases

Fingerprint Dive into the research topics of 'Heterologous protection in pigs induced by a plasmid-cured and crp gene-deleted Salmonella choleraesuis live vaccine'. Together they form a unique fingerprint.

Cite this