TY - JOUR
T1 - Hexavalent chromium induced ROS formation, Akt, NF-κB, and MAPK activation, and TNF-α and IL-1α production in keratinocytes
AU - Wang, Bour Jr
AU - Sheu, Hamm Ming
AU - Guo, Yue Liang
AU - Lee, Yu Hsuan
AU - Lai, Ching Shu
AU - Pan, Min Hsiung
AU - Wang, Ying Jan
N1 - Funding Information:
The authors are grateful for the comments and suggestions of Tsai-Hsiu Yang, PhD., editorial assistance from Miss Mary Mullan, and technical assistance from Shu-Huei Lin. This study was supported by the National Science Council , Taiwan ( NSC 98-2314-B-006-034-MY2 ).
Copyright:
Copyright 2010 Elsevier B.V., All rights reserved.
PY - 2010/10
Y1 - 2010/10
N2 - In certain cell types, it has been found that, hexavalent chromium could increase ROS formation, activate cell signaling and stimulate the release of cytokines. But, in keratinocytes, these effects have not yet fully been demonstrated. Our aim is to observe the above effects of hexavalent chromium on keratinocytes. By utilizing HaCaT cells and the skin of albino guinea pigs, we showed that hexavalent chromium could increase ROS formation, activate the Akt, NF-kB, and MAPK pathways as well as increase the production of cytokines, including TNF-α and IL-1α. The release of these cytokines from keratinocytes is considered to be a key participant in the pathogenesis of contact hypersensitivity. Among cement workers, chromium hypersensitivity is an important occupational skin disease issue. Therefore, the observations of our study help us better understand the role of hexavalent chromium on the development of chromium hypersensitivity, which might provide clues for clinicians in the development of chemopreventative agents for the prevention of chromium hypersensitivity among cement workers.
AB - In certain cell types, it has been found that, hexavalent chromium could increase ROS formation, activate cell signaling and stimulate the release of cytokines. But, in keratinocytes, these effects have not yet fully been demonstrated. Our aim is to observe the above effects of hexavalent chromium on keratinocytes. By utilizing HaCaT cells and the skin of albino guinea pigs, we showed that hexavalent chromium could increase ROS formation, activate the Akt, NF-kB, and MAPK pathways as well as increase the production of cytokines, including TNF-α and IL-1α. The release of these cytokines from keratinocytes is considered to be a key participant in the pathogenesis of contact hypersensitivity. Among cement workers, chromium hypersensitivity is an important occupational skin disease issue. Therefore, the observations of our study help us better understand the role of hexavalent chromium on the development of chromium hypersensitivity, which might provide clues for clinicians in the development of chemopreventative agents for the prevention of chromium hypersensitivity among cement workers.
UR - http://www.scopus.com/inward/record.url?scp=77955921323&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=77955921323&partnerID=8YFLogxK
U2 - 10.1016/j.toxlet.2010.06.024
DO - 10.1016/j.toxlet.2010.06.024
M3 - Article
C2 - 20619327
AN - SCOPUS:77955921323
VL - 198
SP - 216
EP - 224
JO - Toxicology Letters
JF - Toxicology Letters
SN - 0378-4274
IS - 2
ER -