HHR23A, a human homolog of Saccharomyces cerevisiae Rad23, regulates xeroderma pigmentosum C protein and is required for nucleotide excision repair

Hui Chuan Hsieh, Yi Hsuan Hsieh, Yu Hsin Huang, Fan Ching Shen, Han Ni Tsai, Jui He Tsai, Yu Ting Lai, Yu Ting Wang, Woei-Jer Chuang, Wen-Ya Huang

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

HHR23A and hHR23B are the human homologs of Saccharomyces cerevisiae Rad23. hHR23B is associated with the nucleotide excision repair (NER) factor xeroderma pigmentosum C (XPC) protein and is required for global genome repair. The function of hHR23A is not yet clear. In this study, the potential function of the hHR23A protein was investigated using RNA interference techniques. The hHR23A knock-down (KD) construct diminished the RNA level of hHR23A protein by approximately 60%, and it did not interfere with expression of the hHR23B gene. Based on Southwestern immunoblot and host-cell reactivation assays, hHR23A KD cells were found to be deficient in DNA repair activity against the DNA damage caused by UVC irradiation. In these hHR23AKD cells, the XPC gene was not normally induced by UVC irradiation, indicating that the hHR23A protein is involved in NER through regulation of the DNA damage recognition protein XPC. Co-immunoprecipitation experiments revealed that hHR23A was associated with a small portion of hHR23B and the majority of p53 protein, indicating that hHR23A regulates the function of XPC by its association with the NER activator p53.

Original languageEnglish
Pages (from-to)181-187
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume335
Issue number1
DOIs
Publication statusPublished - 2005 Sep 16

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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