High efficiency of electroplating Ni-Co nanowires upon the capture of immobilization of histidine-tagged protein

Ching Yuan Ho, Yi Hua Fan, Yaw Jen Chang

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

A Ni-Co compound (9:1) alloy of nanowires (NWs) synthesized by TiO2 nanotube templates was treated by N2/H2 annealing to enhance the immobilization of penta-histidine-tagged (5 x His-tagged) biotin. Based on the theory of immobilized metal affinity chromatography (IMAC), the chelator of transition, including Ni+2 and Co+2 metallic ions, was used to capture the 5 x His-tagged biotin by nanowire structure in this study. Physical properties and electrical resistivity in one dimension of annealed Ni-Co alloy NWs were characterized and then, the protein capture efficiencies were observed by measuring fluorescence intensities. By appropriate treatment on metallic Ni-Co NWs ligand surfaces, it was found that lower electrical resistivity combined with a higher saturation magnetic flux density (Bs) may be useful for immobilizing 5 x His-tag biotins. Furthermore, there is not only a higher contact probability but the surface-to-volume ratio of one dimensional Ni-Co alloy NW ligands proposes higher detected fluorescence sensitivity than that of Ni-Co film.

Original languageEnglish
Pages (from-to)4778-4783
Number of pages6
JournalJournal of Nanoscience and Nanotechnology
Volume17
Issue number7
DOIs
Publication statusPublished - 2017 Jan 1

All Science Journal Classification (ASJC) codes

  • Bioengineering
  • Chemistry(all)
  • Biomedical Engineering
  • Materials Science(all)
  • Condensed Matter Physics

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