High frequency stimulation can suppress globally seizures induced by 4-AP in the rat hippocampus: An acute in vivo study

Chia Chu Chiang, Chou-Ching Lin, Ming-Shaung Ju, Dominique M. Durand

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Background: High frequency stimulation (HFS) on the hippocampus can locally suppress epileptiform activity in-vitro and decrease seizure frequency in vivo. In-vitro HFS on the ventral commissural tract, a novel target, was shown to block the axonal conduction and suppress activity in the CA1 and CA3 neuron. Objective: To study the spatial extent of seizure suppression by HFS applied on the tract and focus site in an in vivo experiment. Methods: Five adult Sprague-Dawley rats were used for the study. Six electrodes were placed on the septal, middle, and temporal hippocampus bilaterally to simultaneously record seizure activity in the entire hippocampus. Seizure activity was induced by injecting 4-aminopyridine (4-AP) into the right middle part of the hippocampus. Following induction, HFS (100 Hz) was applied to the tract and the focus site at 100, 300 and 500 μA. Results: The induced seizure activity was dominated by two patterns, high frequency spiking and pseudo-periodic spikes. Either tract or focus site stimulation could generate suppression of only the pseudo-periodic spikes. The suppression rates were dependent on stimulation amplitude (P < 0.005, chi square test). However, HFS also caused conversion of the seizure pattern. The conversion rates increased with higher stimulation amplitudes and were higher with focus site stimulation (P < 0.01, Fisher's exact test). Conclusions: The results of this study have two practical implications [1], both tract and focus site stimulation can produce global suppression of hippocampus and [2] the choice of stimulation parameters is critical in order to produce suppression, but not conversion, of seizure pattern.

Original languageEnglish
Pages (from-to)180-189
Number of pages10
JournalBrain Stimulation
Volume6
Issue number2
DOIs
Publication statusPublished - 2013 Mar 1

Fingerprint

4-Aminopyridine
Hippocampus
Seizures
Chi-Square Distribution
Sprague Dawley Rats
Electrodes
Neurons

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)
  • Biophysics
  • Clinical Neurology

Cite this

@article{96a8fc6494bf4c59bdb7433efb3f987c,
title = "High frequency stimulation can suppress globally seizures induced by 4-AP in the rat hippocampus: An acute in vivo study",
abstract = "Background: High frequency stimulation (HFS) on the hippocampus can locally suppress epileptiform activity in-vitro and decrease seizure frequency in vivo. In-vitro HFS on the ventral commissural tract, a novel target, was shown to block the axonal conduction and suppress activity in the CA1 and CA3 neuron. Objective: To study the spatial extent of seizure suppression by HFS applied on the tract and focus site in an in vivo experiment. Methods: Five adult Sprague-Dawley rats were used for the study. Six electrodes were placed on the septal, middle, and temporal hippocampus bilaterally to simultaneously record seizure activity in the entire hippocampus. Seizure activity was induced by injecting 4-aminopyridine (4-AP) into the right middle part of the hippocampus. Following induction, HFS (100 Hz) was applied to the tract and the focus site at 100, 300 and 500 μA. Results: The induced seizure activity was dominated by two patterns, high frequency spiking and pseudo-periodic spikes. Either tract or focus site stimulation could generate suppression of only the pseudo-periodic spikes. The suppression rates were dependent on stimulation amplitude (P < 0.005, chi square test). However, HFS also caused conversion of the seizure pattern. The conversion rates increased with higher stimulation amplitudes and were higher with focus site stimulation (P < 0.01, Fisher's exact test). Conclusions: The results of this study have two practical implications [1], both tract and focus site stimulation can produce global suppression of hippocampus and [2] the choice of stimulation parameters is critical in order to produce suppression, but not conversion, of seizure pattern.",
author = "Chiang, {Chia Chu} and Chou-Ching Lin and Ming-Shaung Ju and Durand, {Dominique M.}",
year = "2013",
month = "3",
day = "1",
doi = "10.1016/j.brs.2012.04.008",
language = "English",
volume = "6",
pages = "180--189",
journal = "Brain Stimulation",
issn = "1935-861X",
publisher = "Elsevier Inc.",
number = "2",

}

High frequency stimulation can suppress globally seizures induced by 4-AP in the rat hippocampus : An acute in vivo study. / Chiang, Chia Chu; Lin, Chou-Ching; Ju, Ming-Shaung; Durand, Dominique M.

In: Brain Stimulation, Vol. 6, No. 2, 01.03.2013, p. 180-189.

Research output: Contribution to journalArticle

TY - JOUR

T1 - High frequency stimulation can suppress globally seizures induced by 4-AP in the rat hippocampus

T2 - An acute in vivo study

AU - Chiang, Chia Chu

AU - Lin, Chou-Ching

AU - Ju, Ming-Shaung

AU - Durand, Dominique M.

PY - 2013/3/1

Y1 - 2013/3/1

N2 - Background: High frequency stimulation (HFS) on the hippocampus can locally suppress epileptiform activity in-vitro and decrease seizure frequency in vivo. In-vitro HFS on the ventral commissural tract, a novel target, was shown to block the axonal conduction and suppress activity in the CA1 and CA3 neuron. Objective: To study the spatial extent of seizure suppression by HFS applied on the tract and focus site in an in vivo experiment. Methods: Five adult Sprague-Dawley rats were used for the study. Six electrodes were placed on the septal, middle, and temporal hippocampus bilaterally to simultaneously record seizure activity in the entire hippocampus. Seizure activity was induced by injecting 4-aminopyridine (4-AP) into the right middle part of the hippocampus. Following induction, HFS (100 Hz) was applied to the tract and the focus site at 100, 300 and 500 μA. Results: The induced seizure activity was dominated by two patterns, high frequency spiking and pseudo-periodic spikes. Either tract or focus site stimulation could generate suppression of only the pseudo-periodic spikes. The suppression rates were dependent on stimulation amplitude (P < 0.005, chi square test). However, HFS also caused conversion of the seizure pattern. The conversion rates increased with higher stimulation amplitudes and were higher with focus site stimulation (P < 0.01, Fisher's exact test). Conclusions: The results of this study have two practical implications [1], both tract and focus site stimulation can produce global suppression of hippocampus and [2] the choice of stimulation parameters is critical in order to produce suppression, but not conversion, of seizure pattern.

AB - Background: High frequency stimulation (HFS) on the hippocampus can locally suppress epileptiform activity in-vitro and decrease seizure frequency in vivo. In-vitro HFS on the ventral commissural tract, a novel target, was shown to block the axonal conduction and suppress activity in the CA1 and CA3 neuron. Objective: To study the spatial extent of seizure suppression by HFS applied on the tract and focus site in an in vivo experiment. Methods: Five adult Sprague-Dawley rats were used for the study. Six electrodes were placed on the septal, middle, and temporal hippocampus bilaterally to simultaneously record seizure activity in the entire hippocampus. Seizure activity was induced by injecting 4-aminopyridine (4-AP) into the right middle part of the hippocampus. Following induction, HFS (100 Hz) was applied to the tract and the focus site at 100, 300 and 500 μA. Results: The induced seizure activity was dominated by two patterns, high frequency spiking and pseudo-periodic spikes. Either tract or focus site stimulation could generate suppression of only the pseudo-periodic spikes. The suppression rates were dependent on stimulation amplitude (P < 0.005, chi square test). However, HFS also caused conversion of the seizure pattern. The conversion rates increased with higher stimulation amplitudes and were higher with focus site stimulation (P < 0.01, Fisher's exact test). Conclusions: The results of this study have two practical implications [1], both tract and focus site stimulation can produce global suppression of hippocampus and [2] the choice of stimulation parameters is critical in order to produce suppression, but not conversion, of seizure pattern.

UR - http://www.scopus.com/inward/record.url?scp=84875210467&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84875210467&partnerID=8YFLogxK

U2 - 10.1016/j.brs.2012.04.008

DO - 10.1016/j.brs.2012.04.008

M3 - Article

C2 - 22621942

AN - SCOPUS:84875210467

VL - 6

SP - 180

EP - 189

JO - Brain Stimulation

JF - Brain Stimulation

SN - 1935-861X

IS - 2

ER -