TY - JOUR
T1 - High-Intensity Focused Ultrasound Attenuates Neural Responses of Sciatic Nerves Isolated from Normal or Neuropathic Rats
AU - Lee, Yee Fun
AU - Lin, Chou Ching
AU - Cheng, Jung Sung
AU - Chen, Gin Shin
N1 - Funding Information:
This study was supported by the National Health Research Institutes (Project ME-102-PP-10).
Publisher Copyright:
© 2015 World Federation for Ultrasound in Medicine & Biology.
PY - 2015/1/1
Y1 - 2015/1/1
N2 - Patients with diabetic neuropathy often have neuropathic pain. The purpose of our work was to investigate the effects of high-intensity focused ultrasound (HIFU) on the conduction block of normal and neuropathic nerves for soothing pain. Adult male Sprague-Dawley rats were used, and diabetes was induced by streptozotocin injection. Diabetic neuropathy was evaluated with animal behavior tests. Sciatic nerves of both control and neuropathic rats were dissected from the starting point of the sciatic nerve to the point where the sural nerve ends near the ankle. The nerves were stored in Ringer's solution. The invitro nerve was placed on a self-developed experimental platform for HIFU exposure. Stimulation and recording of the compound action potentials (CAPs) and sensory action potentials (SAPs) were performed. Control and neuropathic nerves exposed or not exposed to HIFU were submitted to histologic analysis. For the control and neuropathic nerves, suppression of CAPs and SAPs started 2min post-HIFU treatment. Maximum suppression of SAPs was 34.4±3.2% for the control rats and 11.6±2.0% and 9.8±3.0% for rats 4wk post-injection and 8wk post-injection, respectively. Time to full recovery was 25, 70 and 80min, respectively. Histologic analysis revealed that the nerves in which CAPs and SAPs did not fully recover were damaged thermally or mechanically by HIFU. It is feasible to reversibly block nerves with appropriate HIFU treatment. Diabetic nerves were less suppressed by HIFU and were more vulnerable to permanent damage.
AB - Patients with diabetic neuropathy often have neuropathic pain. The purpose of our work was to investigate the effects of high-intensity focused ultrasound (HIFU) on the conduction block of normal and neuropathic nerves for soothing pain. Adult male Sprague-Dawley rats were used, and diabetes was induced by streptozotocin injection. Diabetic neuropathy was evaluated with animal behavior tests. Sciatic nerves of both control and neuropathic rats were dissected from the starting point of the sciatic nerve to the point where the sural nerve ends near the ankle. The nerves were stored in Ringer's solution. The invitro nerve was placed on a self-developed experimental platform for HIFU exposure. Stimulation and recording of the compound action potentials (CAPs) and sensory action potentials (SAPs) were performed. Control and neuropathic nerves exposed or not exposed to HIFU were submitted to histologic analysis. For the control and neuropathic nerves, suppression of CAPs and SAPs started 2min post-HIFU treatment. Maximum suppression of SAPs was 34.4±3.2% for the control rats and 11.6±2.0% and 9.8±3.0% for rats 4wk post-injection and 8wk post-injection, respectively. Time to full recovery was 25, 70 and 80min, respectively. Histologic analysis revealed that the nerves in which CAPs and SAPs did not fully recover were damaged thermally or mechanically by HIFU. It is feasible to reversibly block nerves with appropriate HIFU treatment. Diabetic nerves were less suppressed by HIFU and were more vulnerable to permanent damage.
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U2 - 10.1016/j.ultrasmedbio.2014.08.014
DO - 10.1016/j.ultrasmedbio.2014.08.014
M3 - Article
C2 - 25438842
AN - SCOPUS:84919846458
SN - 0301-5629
VL - 41
SP - 132
EP - 142
JO - Ultrasound in Medicine and Biology
JF - Ultrasound in Medicine and Biology
IS - 1
ER -