High-level production of a thermoacidophilic β-glucosidase from Penicillium citrinum YS40-5 by solid-state fermentation with rice bran

I. Son Ng, Chen Wei Li, Shuang Pi Chan, Jiun Ly Chir, Po Ting Chen, Chii Gong Tong, Su May Yu, Tuan Hua David Ho

Research output: Contribution to journalArticlepeer-review

65 Citations (Scopus)

Abstract

A high yield of β-glucosidase (EC 3.2.1.21) of 159.1 U/g-solid activity on 4-nitrophenyl β-d-glucopyranoside (pNPG) was achieved by rice bran-based solid-state fermentation (SSF) of the recently characterized fungus Penicillium citrinum YS40-5. The enzyme was both thermophilic and acidophilic at the optimized temperature and pH of 70 °C and 5.0, respectively. Over 95% of the original β-glucosidase activity was maintained after a prolonged storage at ambient temperature for 4 weeks. The kinetic parameters Vmax, Km and KI were 85.93 U/mg, 1.2 mM and 17.59 mM with pNPG, and 72.49 U/mg, 32.17 mM and 8.29 mM with cellobiose, respectively. The protein band with β-glucosidase activity was characterized by native PAGE followed by MUG-zymogram analysis, and its identity confirmed by nanoLC-MS/MS. A 3.43-fold synergistic effect by combining this β-glucosidase with Trichoderma reesei cellulases was observed, indicating this enzyme could potentially be used for improving the efficiency of cellulosic bioconversion.

Original languageEnglish
Pages (from-to)1310-1317
Number of pages8
JournalBioresource technology
Volume101
Issue number4
DOIs
Publication statusPublished - 2010 Feb 1

All Science Journal Classification (ASJC) codes

  • Bioengineering
  • Environmental Engineering
  • Renewable Energy, Sustainability and the Environment
  • Waste Management and Disposal

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