TY - JOUR
T1 - High levels of regulatory T cells in blood are a poor prognostic factor in patients with diffuse large B-cell lymphoma
AU - Chang, Chen
AU - Wu, Shang Yin
AU - Kang, Yu Wei
AU - Lin, Kun Piao
AU - Chen, Tsai Yun
AU - Medeiros, L. Jeffrey
AU - Chang, Kung Chao
N1 - Publisher Copyright:
© American Society for Clinical Pathology.
PY - 2015/12
Y1 - 2015/12
N2 - Objectives: Host immunity likely plays a role in preventing progression of diffuse large B-cell lymphoma (DLBCL). Analysis of host immune cells may provide useful information for assessing prognosis or possibly clinical management. Methods: Peripheral blood samples from 77 patients with DLBCL and 30 healthy volunteers were analyzed using flow cytometry immunophenotyping. CBC counts, T-cell subsets, and dendritic cells (DCs) were detected, and the results were correlated with clinicopathologic characteristics. Results: Compared with healthy volunteers, patients with DLBCL had significantly higher leukocyte and monocyte counts (P <.001); higher percentages of neutrophils (P <.001), "natural" regulatory T cells (Tregs; CD3+Foxp3+, P <.001), and immature DCs (CD83-CD1a+, P =.005); and lower percentages of lymphocytes (P <.001) and helper T cells (P =.038). In univariate analysis, high neutrophil counts (≥6,000/μL, P =.014) and "induced" Tregs (CD4+CD25+, P =.026) were poor survival factors along with high International Prognostic Index scores (P <.001) and other high-risk clinical parameters. In multivariate analysis, high Tregs retained significance. Suppression of lymphocytes correlated with poor clinical factors; higher natural Tregs correlated with a lower CD4+/CD8+ ratio (P =.035) and more immature DCs (P =.055). Conclusions: Changes in blood immune cells occur in patients with DLBCL. The results also support a suppressive role of Tregs in adaptive immunity and correlate with poor-risk prognostic factors.
AB - Objectives: Host immunity likely plays a role in preventing progression of diffuse large B-cell lymphoma (DLBCL). Analysis of host immune cells may provide useful information for assessing prognosis or possibly clinical management. Methods: Peripheral blood samples from 77 patients with DLBCL and 30 healthy volunteers were analyzed using flow cytometry immunophenotyping. CBC counts, T-cell subsets, and dendritic cells (DCs) were detected, and the results were correlated with clinicopathologic characteristics. Results: Compared with healthy volunteers, patients with DLBCL had significantly higher leukocyte and monocyte counts (P <.001); higher percentages of neutrophils (P <.001), "natural" regulatory T cells (Tregs; CD3+Foxp3+, P <.001), and immature DCs (CD83-CD1a+, P =.005); and lower percentages of lymphocytes (P <.001) and helper T cells (P =.038). In univariate analysis, high neutrophil counts (≥6,000/μL, P =.014) and "induced" Tregs (CD4+CD25+, P =.026) were poor survival factors along with high International Prognostic Index scores (P <.001) and other high-risk clinical parameters. In multivariate analysis, high Tregs retained significance. Suppression of lymphocytes correlated with poor clinical factors; higher natural Tregs correlated with a lower CD4+/CD8+ ratio (P =.035) and more immature DCs (P =.055). Conclusions: Changes in blood immune cells occur in patients with DLBCL. The results also support a suppressive role of Tregs in adaptive immunity and correlate with poor-risk prognostic factors.
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U2 - 10.1309/AJCPUJGMVV6ZF4GG
DO - 10.1309/AJCPUJGMVV6ZF4GG
M3 - Article
C2 - 26573001
AN - SCOPUS:84947919322
SN - 0002-9173
VL - 144
SP - 935
EP - 944
JO - American Journal of Clinical Pathology
JF - American Journal of Clinical Pathology
IS - 6
ER -