High prevalence of human papillomavirus types 16 and 18 in middle-ear carcinomas

Sen Tien Tsai, Ching Li, Ying Tai Jin, Wen Yuan Chao, Ih Jen Su

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29 Citations (Scopus)


Chronic suppurative otitis media, averaging 20 or more years of duration, has been associated with cancer in this region in 40%-80% of cases. Although human papillomaviruses (HPV) have been implicated in many human squamous-cell neoplasms, their role in the pathogenesis of middle-ear malignancies remains unexplored. In this study, we investigated the presence and subtypes of HPV in middle-ear carcinomas. Formalin-fixed and paraffin- embedded tumor tissues were sampled for DNA extraction. PCR was done with consensus primers, capable of detecting HPV 16, 18, 31, 33, 52b and 58. Typing of the products generated by consensus primers was performed with restriction enzyme digestion. It was found that a resulting 89% (8/9) of the middle-ear carcinomas contained HPV DNA. Coexistence of HPV 16 and 18 was detected in 3 squamous-cell carcinomas. HPV 16 was detected in 4 squamous- cell carcinomas and I adenocarcinoma. The high prevalence of high-risk-type HPV in carcinomas of the middle ear suggests that viral infection may be an important etiologic component in the carcinogenic process.

Original languageEnglish
Pages (from-to)208-212
Number of pages5
JournalInternational Journal of Cancer
Issue number2
Publication statusPublished - 1997

All Science Journal Classification (ASJC) codes

  • Oncology
  • Cancer Research


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