High-speed label-free functional photoacoustic microscopy of mouse brain in action

Junjie Yao, Lidai Wang, Joon Mo Yang, Konstantin I. Maslov, Terence T.W. Wong, Lei Li, Chih Hsien Huang, Jun Zou, Lihong V. Wang

Research output: Contribution to journalArticlepeer-review

380 Citations (Scopus)

Abstract

We present fast functional photoacoustic microscopy (PAM) for three-dimensional high-resolution, high-speed imaging of the mouse brain, complementary to other imaging modalities. We implemented a single-wavelength pulse-width-based method with a one-dimensional imaging rate of 100 kHz to image blood oxygenation with capillary-level resolution. We applied PAM to image the vascular morphology, blood oxygenation, blood flow and oxygen metabolism in both resting and stimulated states in the mouse brain.

Original languageEnglish
Pages (from-to)407-410
Number of pages4
JournalNature Methods
Volume12
Issue number5
DOIs
Publication statusPublished - 2015 Apr 29

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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