High-throughput electrokinetic bioparticle focusing based on a travelling-wave dielectrophoretic field

I. Fang Cheng, Cheng Che Chung, Hsien-Chang Chang

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

This study presents a sheathless and portable microfluidic chip that is capable of high-throughput focusing bioparticles based on 3D travelling-wave dielectrophoresis (twDEP). High-throughput focusing is achieved by sustaining a centralized twDEP field normal to the continuous through-flow direction. Two twDEP electrode arrays are formed from upper and lower walls of the microchannel and extend to its center, which induce twDEP forces to provide the lateral displacements in two directions for focusing the bioparticles. Bioparticles can be focused to the center of the microchannel effectively by twDEP conveyance when the characteristic time due to twDEP conveying in the y direction is shorter than the residence time of the particles within twDEP electrode array. Red blood cells can be effectively focused into a narrow particle stream (∼10 μm) below a critical flow rate of 10 ll/min (linear flow velocity ∼5 mm/s), when under a voltage of 14 Vp-p at a frequency of 500 kHz is applied. Approximately 90% focusing efficiency for red blood cells can be achieved within two 6-mm-long electrode arrays when the flow rate is below 12 μl/min. Blood cells and Candida cells were also focused and sorted successfully based on their different twDEP mobilities. Compared to conventional 3D-paired DEP focusing, velocity is enhanced nearly four folds of magnitude. 3D twDEP provides the lateral displacements of particles and long residence time for migrating particles in a high-speed continuous flow, which breaks through the limitation of many electrokinetic cell manipulation techniques.

Original languageEnglish
Pages (from-to)649-660
Number of pages12
JournalMicrofluidics and Nanofluidics
Volume10
Issue number3
DOIs
Publication statusPublished - 2011 Mar 1

Fingerprint

electrokinetics
Electrophoresis
traveling waves
Throughput
Blood
flow velocity
Cells
erythrocytes
microchannels
Microchannels
Electrodes
electrodes
Flow rate
critical flow
blood cells
Candida
sustaining
Conveying
cells
Microfluidics

All Science Journal Classification (ASJC) codes

  • Condensed Matter Physics
  • Electronic, Optical and Magnetic Materials
  • Materials Chemistry

Cite this

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abstract = "This study presents a sheathless and portable microfluidic chip that is capable of high-throughput focusing bioparticles based on 3D travelling-wave dielectrophoresis (twDEP). High-throughput focusing is achieved by sustaining a centralized twDEP field normal to the continuous through-flow direction. Two twDEP electrode arrays are formed from upper and lower walls of the microchannel and extend to its center, which induce twDEP forces to provide the lateral displacements in two directions for focusing the bioparticles. Bioparticles can be focused to the center of the microchannel effectively by twDEP conveyance when the characteristic time due to twDEP conveying in the y direction is shorter than the residence time of the particles within twDEP electrode array. Red blood cells can be effectively focused into a narrow particle stream (∼10 μm) below a critical flow rate of 10 ll/min (linear flow velocity ∼5 mm/s), when under a voltage of 14 Vp-p at a frequency of 500 kHz is applied. Approximately 90{\%} focusing efficiency for red blood cells can be achieved within two 6-mm-long electrode arrays when the flow rate is below 12 μl/min. Blood cells and Candida cells were also focused and sorted successfully based on their different twDEP mobilities. Compared to conventional 3D-paired DEP focusing, velocity is enhanced nearly four folds of magnitude. 3D twDEP provides the lateral displacements of particles and long residence time for migrating particles in a high-speed continuous flow, which breaks through the limitation of many electrokinetic cell manipulation techniques.",
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High-throughput electrokinetic bioparticle focusing based on a travelling-wave dielectrophoretic field. / Cheng, I. Fang; Chung, Cheng Che; Chang, Hsien-Chang.

In: Microfluidics and Nanofluidics, Vol. 10, No. 3, 01.03.2011, p. 649-660.

Research output: Contribution to journalArticle

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