Higher Motility Enhances Bacterial Density and Inflammatory Response in Dyspeptic Patients Infected with Helicobacter pylori

Cheng Yen Kao, Bor Shyang Sheu, Shew Meei Sheu, Hsiao Bai Yang, Wei Lun Chang, Hsiu Chi Cheng, Jiunn Jong Wu

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Background: Motility mediated by the flagella of Helicobacter pylori is important for the cells to move toward the gastric mucus in niches adjacent to the epithelium; then, H. pylori uses the adhesin SabA to interact with sialyl-Lex on inflammatory host cells for persistent infection. Here, we reveal the clinical association of bacterial motility, SabA expression, and pathological outcomes. Methods: Ninety-six clinical isolates were screened for bacterial motility, and the expression of SabA of each isolate was confirmed by Western blotting. H. pylori-infected patients were assessed for their bacterial density, sialyl-Lex expression, inflammatory scores, and clinical diseases. Results: The mean diameter in the motility assay was 17 mm, and eight (8.3%) of the strains had impaired motility, with a diameter <5 mm. H. pylori density in cardia, the acute inflammatory score in the body locus, and the prevalence rate of gastric atrophy were increased in patients infected with higher-motility strains (p = .023, <.001, or <.001, respectively). The total inflammatory scores (both acute and chronic) and bacterial density dramatically increased in patients expressing the sialyl-Lex antigen and infected with higher-motility, SabA-positive H. pylori (p = .016, .01, or .005, respectively). Conclusion: These results suggest that the higher motility of H. pylori enhances pathological outcomes, and the SabA-sialyl-Lex interaction has a synergistic effect on virulence of the higher-motility strains.

Original languageEnglish
Pages (from-to)411-416
Number of pages6
JournalHelicobacter
Volume17
Issue number6
DOIs
Publication statusPublished - 2012 Dec 1

Fingerprint

Helicobacter pylori
Stomach
Cardia
Flagella
Mucus
Atrophy
Virulence
Epithelium
Western Blotting
Antigens
5-acetylneuraminyl-(2-3)-galactosyl-(1-4)-(fucopyranosyl-(1-3))-N-acetylglucosamine
Infection

All Science Journal Classification (ASJC) codes

  • Gastroenterology
  • Infectious Diseases

Cite this

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title = "Higher Motility Enhances Bacterial Density and Inflammatory Response in Dyspeptic Patients Infected with Helicobacter pylori",
abstract = "Background: Motility mediated by the flagella of Helicobacter pylori is important for the cells to move toward the gastric mucus in niches adjacent to the epithelium; then, H. pylori uses the adhesin SabA to interact with sialyl-Lex on inflammatory host cells for persistent infection. Here, we reveal the clinical association of bacterial motility, SabA expression, and pathological outcomes. Methods: Ninety-six clinical isolates were screened for bacterial motility, and the expression of SabA of each isolate was confirmed by Western blotting. H. pylori-infected patients were assessed for their bacterial density, sialyl-Lex expression, inflammatory scores, and clinical diseases. Results: The mean diameter in the motility assay was 17 mm, and eight (8.3{\%}) of the strains had impaired motility, with a diameter <5 mm. H. pylori density in cardia, the acute inflammatory score in the body locus, and the prevalence rate of gastric atrophy were increased in patients infected with higher-motility strains (p = .023, <.001, or <.001, respectively). The total inflammatory scores (both acute and chronic) and bacterial density dramatically increased in patients expressing the sialyl-Lex antigen and infected with higher-motility, SabA-positive H. pylori (p = .016, .01, or .005, respectively). Conclusion: These results suggest that the higher motility of H. pylori enhances pathological outcomes, and the SabA-sialyl-Lex interaction has a synergistic effect on virulence of the higher-motility strains.",
author = "Kao, {Cheng Yen} and Sheu, {Bor Shyang} and Sheu, {Shew Meei} and Yang, {Hsiao Bai} and Chang, {Wei Lun} and Cheng, {Hsiu Chi} and Wu, {Jiunn Jong}",
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Higher Motility Enhances Bacterial Density and Inflammatory Response in Dyspeptic Patients Infected with Helicobacter pylori. / Kao, Cheng Yen; Sheu, Bor Shyang; Sheu, Shew Meei; Yang, Hsiao Bai; Chang, Wei Lun; Cheng, Hsiu Chi; Wu, Jiunn Jong.

In: Helicobacter, Vol. 17, No. 6, 01.12.2012, p. 411-416.

Research output: Contribution to journalArticle

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T1 - Higher Motility Enhances Bacterial Density and Inflammatory Response in Dyspeptic Patients Infected with Helicobacter pylori

AU - Kao, Cheng Yen

AU - Sheu, Bor Shyang

AU - Sheu, Shew Meei

AU - Yang, Hsiao Bai

AU - Chang, Wei Lun

AU - Cheng, Hsiu Chi

AU - Wu, Jiunn Jong

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N2 - Background: Motility mediated by the flagella of Helicobacter pylori is important for the cells to move toward the gastric mucus in niches adjacent to the epithelium; then, H. pylori uses the adhesin SabA to interact with sialyl-Lex on inflammatory host cells for persistent infection. Here, we reveal the clinical association of bacterial motility, SabA expression, and pathological outcomes. Methods: Ninety-six clinical isolates were screened for bacterial motility, and the expression of SabA of each isolate was confirmed by Western blotting. H. pylori-infected patients were assessed for their bacterial density, sialyl-Lex expression, inflammatory scores, and clinical diseases. Results: The mean diameter in the motility assay was 17 mm, and eight (8.3%) of the strains had impaired motility, with a diameter <5 mm. H. pylori density in cardia, the acute inflammatory score in the body locus, and the prevalence rate of gastric atrophy were increased in patients infected with higher-motility strains (p = .023, <.001, or <.001, respectively). The total inflammatory scores (both acute and chronic) and bacterial density dramatically increased in patients expressing the sialyl-Lex antigen and infected with higher-motility, SabA-positive H. pylori (p = .016, .01, or .005, respectively). Conclusion: These results suggest that the higher motility of H. pylori enhances pathological outcomes, and the SabA-sialyl-Lex interaction has a synergistic effect on virulence of the higher-motility strains.

AB - Background: Motility mediated by the flagella of Helicobacter pylori is important for the cells to move toward the gastric mucus in niches adjacent to the epithelium; then, H. pylori uses the adhesin SabA to interact with sialyl-Lex on inflammatory host cells for persistent infection. Here, we reveal the clinical association of bacterial motility, SabA expression, and pathological outcomes. Methods: Ninety-six clinical isolates were screened for bacterial motility, and the expression of SabA of each isolate was confirmed by Western blotting. H. pylori-infected patients were assessed for their bacterial density, sialyl-Lex expression, inflammatory scores, and clinical diseases. Results: The mean diameter in the motility assay was 17 mm, and eight (8.3%) of the strains had impaired motility, with a diameter <5 mm. H. pylori density in cardia, the acute inflammatory score in the body locus, and the prevalence rate of gastric atrophy were increased in patients infected with higher-motility strains (p = .023, <.001, or <.001, respectively). The total inflammatory scores (both acute and chronic) and bacterial density dramatically increased in patients expressing the sialyl-Lex antigen and infected with higher-motility, SabA-positive H. pylori (p = .016, .01, or .005, respectively). Conclusion: These results suggest that the higher motility of H. pylori enhances pathological outcomes, and the SabA-sialyl-Lex interaction has a synergistic effect on virulence of the higher-motility strains.

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