Histopathologic changes in kidney and liver correlate with streptococcal pyrogenic exotoxin B production in the mouse model of group A streptococcal infection

Chih Feng Kuo, Yueh Hsia Luo, Hsiu Yueh Lin, Kuen Jeng Huang, Jiunn Jong Wu, Huan Yao Lei, Ming T. Lin, Woei-Jer Chuang, Ching-Chuan Liu, Ying Tai Jin, Yee-Shin Lin

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Abstract

Previous studies show that isogenic mutants deficient in streptococcal pyrogenic exotoxin B (SPE B) cause less mortality and skin tissue damage than wild-type strains of Streptococcus pyogenes when inoculated into mice via an air pouch. In this study, the growth and dissemination of bacteria, pathologic changes in various organs, and their correlation with SPE B production were examined. Bacterial numbers in the air pouch from wild-type strain NZ131-infected mice increased at 48 h, while those from speB mutant SW510-infected mice continuously reduced. Mice infected with NZ131 developed bacteremia and greater dissemination in the kidney, liver, and spleen; those infected with SW510 showed either no or slight bacteremia and dissemination. Co-inoculation of SW510 with recombinant SPE B showed a higher bacterial count in the air pouch, bacteremia, and organ dissemination compared to co-inoculation with a C192S mutant lacking protease activity. The histopathologic changes examined showed lesions in kidney and liver in the NZ131-infected but not in SW510-infected mice. The elevation in sera of BUN, AST, and ALT correlated positively with renal and liver impairment. Taken together, SPE B produced during S. pyogenes infection plays a pathogenic role. A direct effect of SPE B on vessel permeability change was also demonstrated.

Original languageEnglish
Pages (from-to)273-285
Number of pages13
JournalMicrobial Pathogenesis
Volume36
Issue number5
DOIs
Publication statusPublished - 2004 May 1

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Streptococcal Infections
Kidney
Bacteremia
Liver
Streptococcus pyogenes
Air
Bacterial Load
Blood Urea Nitrogen
Permeability
Peptide Hydrolases
Spleen
erythrogenic toxin
Bacteria
Skin
Mortality
Growth
Infection
Serum

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Infectious Diseases

Cite this

Kuo, Chih Feng ; Luo, Yueh Hsia ; Lin, Hsiu Yueh ; Huang, Kuen Jeng ; Wu, Jiunn Jong ; Lei, Huan Yao ; Lin, Ming T. ; Chuang, Woei-Jer ; Liu, Ching-Chuan ; Jin, Ying Tai ; Lin, Yee-Shin. / Histopathologic changes in kidney and liver correlate with streptococcal pyrogenic exotoxin B production in the mouse model of group A streptococcal infection. In: Microbial Pathogenesis. 2004 ; Vol. 36, No. 5. pp. 273-285.
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abstract = "Previous studies show that isogenic mutants deficient in streptococcal pyrogenic exotoxin B (SPE B) cause less mortality and skin tissue damage than wild-type strains of Streptococcus pyogenes when inoculated into mice via an air pouch. In this study, the growth and dissemination of bacteria, pathologic changes in various organs, and their correlation with SPE B production were examined. Bacterial numbers in the air pouch from wild-type strain NZ131-infected mice increased at 48 h, while those from speB mutant SW510-infected mice continuously reduced. Mice infected with NZ131 developed bacteremia and greater dissemination in the kidney, liver, and spleen; those infected with SW510 showed either no or slight bacteremia and dissemination. Co-inoculation of SW510 with recombinant SPE B showed a higher bacterial count in the air pouch, bacteremia, and organ dissemination compared to co-inoculation with a C192S mutant lacking protease activity. The histopathologic changes examined showed lesions in kidney and liver in the NZ131-infected but not in SW510-infected mice. The elevation in sera of BUN, AST, and ALT correlated positively with renal and liver impairment. Taken together, SPE B produced during S. pyogenes infection plays a pathogenic role. A direct effect of SPE B on vessel permeability change was also demonstrated.",
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Histopathologic changes in kidney and liver correlate with streptococcal pyrogenic exotoxin B production in the mouse model of group A streptococcal infection. / Kuo, Chih Feng; Luo, Yueh Hsia; Lin, Hsiu Yueh; Huang, Kuen Jeng; Wu, Jiunn Jong; Lei, Huan Yao; Lin, Ming T.; Chuang, Woei-Jer; Liu, Ching-Chuan; Jin, Ying Tai; Lin, Yee-Shin.

In: Microbial Pathogenesis, Vol. 36, No. 5, 01.05.2004, p. 273-285.

Research output: Contribution to journalArticle

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