HLH-30/TFEB-mediated autophagy functions in a cell-autonomous manner for epithelium intrinsic cellular defense against bacterial pore-forming toxin in C. elegans

Huan Da Chen, Cheng Yuan Kao, Bang Yu Liu, Shin Whei Huang, Cheng Ju Kuo, Jhen-Wei Ruan, Yen Hung Lin, Cheng Rung Huang, Yu-Hung Chen, Horng Dar Wang, Raffi V. Aroian, Chang-Shi Chen

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Autophagy is an evolutionarily conserved intracellular system that maintains cellular homeostasis by degrading and recycling damaged cellular components. The transcription factor HLH-30/TFEB-mediated autophagy has been reported to regulate tolerance to bacterial infection, but less is known about the bona fide bacterial effector that activates HLH-30 and autophagy. Here, we reveal that bacterial membrane pore-forming toxin (PFT) induces autophagy in an HLH-30-dependent manner in Caenorhabditis elegans. Moreover, autophagy controls the susceptibility of animals to PFT toxicity through xenophagic degradation of PFT and repair of membrane-pore cell-autonomously in the PFT-targeted intestinal cells in C. elegans. These results demonstrate that autophagic pathways and autophagy are induced partly at the transcriptional level through HLH-30 activation and are required to protect metazoan upon PFT intoxication. Together, our data show a new and powerful connection between HLH-30-mediated autophagy and epithelium intrinsic cellular defense against the single most common mode of bacterial attack in vivo.

Original languageEnglish
Pages (from-to)371-385
Number of pages15
JournalAutophagy
Volume13
Issue number2
DOIs
Publication statusPublished - 2017 Feb 1

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Autophagy
Epithelium
Caenorhabditis elegans
Immunotoxins
Recycling
Bacterial Infections
Homeostasis
Transcription Factors
Cell Membrane
Membranes

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Cell Biology

Cite this

Chen, Huan Da ; Kao, Cheng Yuan ; Liu, Bang Yu ; Huang, Shin Whei ; Kuo, Cheng Ju ; Ruan, Jhen-Wei ; Lin, Yen Hung ; Huang, Cheng Rung ; Chen, Yu-Hung ; Wang, Horng Dar ; Aroian, Raffi V. ; Chen, Chang-Shi. / HLH-30/TFEB-mediated autophagy functions in a cell-autonomous manner for epithelium intrinsic cellular defense against bacterial pore-forming toxin in C. elegans. In: Autophagy. 2017 ; Vol. 13, No. 2. pp. 371-385.
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HLH-30/TFEB-mediated autophagy functions in a cell-autonomous manner for epithelium intrinsic cellular defense against bacterial pore-forming toxin in C. elegans. / Chen, Huan Da; Kao, Cheng Yuan; Liu, Bang Yu; Huang, Shin Whei; Kuo, Cheng Ju; Ruan, Jhen-Wei; Lin, Yen Hung; Huang, Cheng Rung; Chen, Yu-Hung; Wang, Horng Dar; Aroian, Raffi V.; Chen, Chang-Shi.

In: Autophagy, Vol. 13, No. 2, 01.02.2017, p. 371-385.

Research output: Contribution to journalArticle

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