HLH-30/TFEB-mediated autophagy functions in a cell-autonomous manner for epithelium intrinsic cellular defense against bacterial pore-forming toxin in C. elegans

Huan Da Chen, Cheng Yuan Kao, Bang Yu Liu, Shin Whei Huang, Cheng Ju Kuo, Jhen Wei Ruan, Yen Hung Lin, Cheng Rung Huang, Yu Hung Chen, Horng Dar Wang, Raffi V. Aroian, Chang Shi Chen

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Autophagy is an evolutionarily conserved intracellular system that maintains cellular homeostasis by degrading and recycling damaged cellular components. The transcription factor HLH-30/TFEB-mediated autophagy has been reported to regulate tolerance to bacterial infection, but less is known about the bona fide bacterial effector that activates HLH-30 and autophagy. Here, we reveal that bacterial membrane pore-forming toxin (PFT) induces autophagy in an HLH-30-dependent manner in Caenorhabditis elegans. Moreover, autophagy controls the susceptibility of animals to PFT toxicity through xenophagic degradation of PFT and repair of membrane-pore cell-autonomously in the PFT-targeted intestinal cells in C. elegans. These results demonstrate that autophagic pathways and autophagy are induced partly at the transcriptional level through HLH-30 activation and are required to protect metazoan upon PFT intoxication. Together, our data show a new and powerful connection between HLH-30-mediated autophagy and epithelium intrinsic cellular defense against the single most common mode of bacterial attack in vivo.

Original languageEnglish
Pages (from-to)371-385
Number of pages15
JournalAutophagy
Volume13
Issue number2
DOIs
Publication statusPublished - 2017 Feb 1

Fingerprint

Autophagy
Epithelium
Caenorhabditis elegans
Immunotoxins
Recycling
Bacterial Infections
Homeostasis
Transcription Factors
Cell Membrane
Membranes

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Cell Biology

Cite this

Chen, Huan Da ; Kao, Cheng Yuan ; Liu, Bang Yu ; Huang, Shin Whei ; Kuo, Cheng Ju ; Ruan, Jhen Wei ; Lin, Yen Hung ; Huang, Cheng Rung ; Chen, Yu Hung ; Wang, Horng Dar ; Aroian, Raffi V. ; Chen, Chang Shi. / HLH-30/TFEB-mediated autophagy functions in a cell-autonomous manner for epithelium intrinsic cellular defense against bacterial pore-forming toxin in C. elegans. In: Autophagy. 2017 ; Vol. 13, No. 2. pp. 371-385.
@article{d2c01293a85843e4857c9a8c2bb659da,
title = "HLH-30/TFEB-mediated autophagy functions in a cell-autonomous manner for epithelium intrinsic cellular defense against bacterial pore-forming toxin in C. elegans",
abstract = "Autophagy is an evolutionarily conserved intracellular system that maintains cellular homeostasis by degrading and recycling damaged cellular components. The transcription factor HLH-30/TFEB-mediated autophagy has been reported to regulate tolerance to bacterial infection, but less is known about the bona fide bacterial effector that activates HLH-30 and autophagy. Here, we reveal that bacterial membrane pore-forming toxin (PFT) induces autophagy in an HLH-30-dependent manner in Caenorhabditis elegans. Moreover, autophagy controls the susceptibility of animals to PFT toxicity through xenophagic degradation of PFT and repair of membrane-pore cell-autonomously in the PFT-targeted intestinal cells in C. elegans. These results demonstrate that autophagic pathways and autophagy are induced partly at the transcriptional level through HLH-30 activation and are required to protect metazoan upon PFT intoxication. Together, our data show a new and powerful connection between HLH-30-mediated autophagy and epithelium intrinsic cellular defense against the single most common mode of bacterial attack in vivo.",
author = "Chen, {Huan Da} and Kao, {Cheng Yuan} and Liu, {Bang Yu} and Huang, {Shin Whei} and Kuo, {Cheng Ju} and Ruan, {Jhen Wei} and Lin, {Yen Hung} and Huang, {Cheng Rung} and Chen, {Yu Hung} and Wang, {Horng Dar} and Aroian, {Raffi V.} and Chen, {Chang Shi}",
year = "2017",
month = "2",
day = "1",
doi = "10.1080/15548627.2016.1256933",
language = "English",
volume = "13",
pages = "371--385",
journal = "Autophagy",
issn = "1554-8627",
publisher = "Landes Bioscience",
number = "2",

}

HLH-30/TFEB-mediated autophagy functions in a cell-autonomous manner for epithelium intrinsic cellular defense against bacterial pore-forming toxin in C. elegans. / Chen, Huan Da; Kao, Cheng Yuan; Liu, Bang Yu; Huang, Shin Whei; Kuo, Cheng Ju; Ruan, Jhen Wei; Lin, Yen Hung; Huang, Cheng Rung; Chen, Yu Hung; Wang, Horng Dar; Aroian, Raffi V.; Chen, Chang Shi.

In: Autophagy, Vol. 13, No. 2, 01.02.2017, p. 371-385.

Research output: Contribution to journalArticle

TY - JOUR

T1 - HLH-30/TFEB-mediated autophagy functions in a cell-autonomous manner for epithelium intrinsic cellular defense against bacterial pore-forming toxin in C. elegans

AU - Chen, Huan Da

AU - Kao, Cheng Yuan

AU - Liu, Bang Yu

AU - Huang, Shin Whei

AU - Kuo, Cheng Ju

AU - Ruan, Jhen Wei

AU - Lin, Yen Hung

AU - Huang, Cheng Rung

AU - Chen, Yu Hung

AU - Wang, Horng Dar

AU - Aroian, Raffi V.

AU - Chen, Chang Shi

PY - 2017/2/1

Y1 - 2017/2/1

N2 - Autophagy is an evolutionarily conserved intracellular system that maintains cellular homeostasis by degrading and recycling damaged cellular components. The transcription factor HLH-30/TFEB-mediated autophagy has been reported to regulate tolerance to bacterial infection, but less is known about the bona fide bacterial effector that activates HLH-30 and autophagy. Here, we reveal that bacterial membrane pore-forming toxin (PFT) induces autophagy in an HLH-30-dependent manner in Caenorhabditis elegans. Moreover, autophagy controls the susceptibility of animals to PFT toxicity through xenophagic degradation of PFT and repair of membrane-pore cell-autonomously in the PFT-targeted intestinal cells in C. elegans. These results demonstrate that autophagic pathways and autophagy are induced partly at the transcriptional level through HLH-30 activation and are required to protect metazoan upon PFT intoxication. Together, our data show a new and powerful connection between HLH-30-mediated autophagy and epithelium intrinsic cellular defense against the single most common mode of bacterial attack in vivo.

AB - Autophagy is an evolutionarily conserved intracellular system that maintains cellular homeostasis by degrading and recycling damaged cellular components. The transcription factor HLH-30/TFEB-mediated autophagy has been reported to regulate tolerance to bacterial infection, but less is known about the bona fide bacterial effector that activates HLH-30 and autophagy. Here, we reveal that bacterial membrane pore-forming toxin (PFT) induces autophagy in an HLH-30-dependent manner in Caenorhabditis elegans. Moreover, autophagy controls the susceptibility of animals to PFT toxicity through xenophagic degradation of PFT and repair of membrane-pore cell-autonomously in the PFT-targeted intestinal cells in C. elegans. These results demonstrate that autophagic pathways and autophagy are induced partly at the transcriptional level through HLH-30 activation and are required to protect metazoan upon PFT intoxication. Together, our data show a new and powerful connection between HLH-30-mediated autophagy and epithelium intrinsic cellular defense against the single most common mode of bacterial attack in vivo.

UR - http://www.scopus.com/inward/record.url?scp=85006164589&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85006164589&partnerID=8YFLogxK

U2 - 10.1080/15548627.2016.1256933

DO - 10.1080/15548627.2016.1256933

M3 - Article

C2 - 27875098

AN - SCOPUS:85006164589

VL - 13

SP - 371

EP - 385

JO - Autophagy

JF - Autophagy

SN - 1554-8627

IS - 2

ER -