Honeycomb-shaped magnetic multilayer thin films for cell trapping

Chen Yu Huang; Wei Chieh Chang; Kun Chieh Yeh; Han Yi Tseng; Ming Shinn Hsu; Jiann Yeu Chen; Zung Hang Wei

doi:10.1039/c6ra01757f

Honeycomb-shaped magnetic multilayer thin films for cell trapping

Chen Yu Huang, Wei Chieh Chang, Kun Chieh Yeh, Han Yi Tseng, Ming Shinn Hsu, Jiann Yeu Chen, Zung Hang Wei

Department of Medical Laboratory Science and Biotechnology

Research output: Contribution to journal › Article › peer-review

4 Citations (Scopus)

Abstract

Honeycomb-shaped magnetic thin films with domain wall (DW) pinning geometry are designed to actively trap magnetically labeled cells. After an initial in-plane magnetic field (H_initial) is applied and later reduced to zero, the resultant magnetization became locally aligned. Human hepatocellular liver carcinoma cell line (HepG2) stably expresses green fluorescent protein (GFP) and is magnetically labeled with superparamagnetic magnetic nanoparticles (MNPs). Prussian blue stain and single cell magnetophoresis are performed to evaluate the internalization of the MNPs. Magnetically labeled cells are then trapped by the stray fields of head-to-head DWs (HH DWs) or tail-to-tail DWs (TT DWs). After co-culturing with magnetic structure, HepG2 cells stretched out and showed filopodia-like protrusions to make contact with adjacent cells.

Original language	English
Pages (from-to)	24299-24303
Number of pages	5
Journal	RSC Advances
Volume	6
Issue number	29
DOIs	https://doi.org/10.1039/c6ra01757f
Publication status	Published - 2016 Feb 1

All Science Journal Classification (ASJC) codes

General Chemistry
General Chemical Engineering

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10.1039/c6ra01757f

Cite this

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title = "Honeycomb-shaped magnetic multilayer thin films for cell trapping",

abstract = "Honeycomb-shaped magnetic thin films with domain wall (DW) pinning geometry are designed to actively trap magnetically labeled cells. After an initial in-plane magnetic field (Hinitial) is applied and later reduced to zero, the resultant magnetization became locally aligned. Human hepatocellular liver carcinoma cell line (HepG2) stably expresses green fluorescent protein (GFP) and is magnetically labeled with superparamagnetic magnetic nanoparticles (MNPs). Prussian blue stain and single cell magnetophoresis are performed to evaluate the internalization of the MNPs. Magnetically labeled cells are then trapped by the stray fields of head-to-head DWs (HH DWs) or tail-to-tail DWs (TT DWs). After co-culturing with magnetic structure, HepG2 cells stretched out and showed filopodia-like protrusions to make contact with adjacent cells.",

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AU - Huang, Chen Yu

AU - Chang, Wei Chieh

AU - Yeh, Kun Chieh

AU - Tseng, Han Yi

AU - Hsu, Ming Shinn

AU - Chen, Jiann Yeu

AU - Wei, Zung Hang

PY - 2016/2/1

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N2 - Honeycomb-shaped magnetic thin films with domain wall (DW) pinning geometry are designed to actively trap magnetically labeled cells. After an initial in-plane magnetic field (Hinitial) is applied and later reduced to zero, the resultant magnetization became locally aligned. Human hepatocellular liver carcinoma cell line (HepG2) stably expresses green fluorescent protein (GFP) and is magnetically labeled with superparamagnetic magnetic nanoparticles (MNPs). Prussian blue stain and single cell magnetophoresis are performed to evaluate the internalization of the MNPs. Magnetically labeled cells are then trapped by the stray fields of head-to-head DWs (HH DWs) or tail-to-tail DWs (TT DWs). After co-culturing with magnetic structure, HepG2 cells stretched out and showed filopodia-like protrusions to make contact with adjacent cells.

AB - Honeycomb-shaped magnetic thin films with domain wall (DW) pinning geometry are designed to actively trap magnetically labeled cells. After an initial in-plane magnetic field (Hinitial) is applied and later reduced to zero, the resultant magnetization became locally aligned. Human hepatocellular liver carcinoma cell line (HepG2) stably expresses green fluorescent protein (GFP) and is magnetically labeled with superparamagnetic magnetic nanoparticles (MNPs). Prussian blue stain and single cell magnetophoresis are performed to evaluate the internalization of the MNPs. Magnetically labeled cells are then trapped by the stray fields of head-to-head DWs (HH DWs) or tail-to-tail DWs (TT DWs). After co-culturing with magnetic structure, HepG2 cells stretched out and showed filopodia-like protrusions to make contact with adjacent cells.

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Honeycomb-shaped magnetic multilayer thin films for cell trapping

Abstract

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