@article{1291511d44af4512b53d469547e88668,
title = "Host CDK-1 and formin mediate microvillar effacement induced by enterohemorrhagic Escherichia coli",
abstract = "Enterohemorrhagic Escherichia coli (EHEC) induces changes to the intestinal cell cytoskeleton and formation of attaching and effacing lesions, characterized by the effacement of microvilli and then formation of actin pedestals to which the bacteria are tightly attached. Here, we use a Caenorhabditis elegans model of EHEC infection to show that microvillar effacement is mediated by a signalling pathway including mitotic cyclin-dependent kinase 1 (CDK1) and diaphanous-related formin 1 (CYK1). Similar observations are also made using EHEC-infected human intestinal cells in vitro. Our results support the use of C. elegans as a host model for studying attaching and effacing lesions in vivo, and reveal that the CDK1-formin signal axis is necessary for EHEC-induced microvillar effacement.",
author = "Huang, {Cheng Rung} and Kuo, {Cheng Ju} and Huang, {Chih Wen} and Chen, {Yu Ting} and Liu, {Bang Yu} and Lee, {Chung Ta} and Chen, {Po Lin} and Chang, {Wen Tsan} and Chen, {Yun Wen} and Lee, {Tzer Min} and Hsieh, {Hui Chen} and Chen, {Chang Shi}",
note = "Funding Information: We thank the Caenorhabditis Genetics Center (CGC), which is supported by the National Institutes of Health (United States) Office of Research Infrastructure Programs (P40 OD010440), for the C. elegans strains. We are grateful to David Pruyne from State University of NY Upstate Medical University for the cyk-1::GFP plasmid. We thank Ken Sato (Gunma University, Japan) for the mCherry::ACT-5 worm. We are grateful to Verena Jantsch (University of Vienna, Austria) for the p-SUN-1ser43 antibody. We are grateful for the assistance from the Taiwan C. elegans core facility (CECF), funded by the Minister of Science and Technology (MOST 108-2319-B-002-004-) Taiwan. We also thank the National RNAi Core Facility at Academia Sinica in Taiwan for providing shRNA reagents and related services. We thank Miranda Loney for editing the manuscript. This work is supported by the Ministry of Science and Technology (MOST) grants (105-2321-B-006-011-, 106-2321-B-006-005-, 107-2628-B-006-003-, and 108-2628-B-006-005-) to CSC. The funder had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. Publisher Copyright: {\textcopyright} 2021, The Author(s).",
year = "2021",
month = dec,
doi = "10.1038/s41467-020-20355-1",
language = "English",
volume = "12",
journal = "Nature Communications",
issn = "2041-1723",
publisher = "Nature Publishing Group",
number = "1",
}