Host CDK-1 and formin mediate microvillar effacement induced by enterohemorrhagic Escherichia coli

Cheng Rung Huang; Cheng Ju Kuo; Chih Wen Huang; Yu Ting Chen; Bang Yu Liu; Chung Ta Lee; Po Lin Chen; Wen Tsan Chang; Yun Wen Chen; Tzer Min Lee; Hui Chen Hsieh; Chang Shi Chen

doi:10.1038/s41467-020-20355-1

Host CDK-1 and formin mediate microvillar effacement induced by enterohemorrhagic Escherichia coli

Cheng Rung Huang, Cheng Ju Kuo, Chih Wen Huang, Yu Ting Chen, Bang Yu Liu, Chung Ta Lee, Po Lin Chen, Wen Tsan Chang, Yun Wen Chen, Tzer Min Lee, Hui Chen Hsieh, Chang Shi Chen

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9 Citations (Scopus)

Abstract

Enterohemorrhagic Escherichia coli (EHEC) induces changes to the intestinal cell cytoskeleton and formation of attaching and effacing lesions, characterized by the effacement of microvilli and then formation of actin pedestals to which the bacteria are tightly attached. Here, we use a Caenorhabditis elegans model of EHEC infection to show that microvillar effacement is mediated by a signalling pathway including mitotic cyclin-dependent kinase 1 (CDK1) and diaphanous-related formin 1 (CYK1). Similar observations are also made using EHEC-infected human intestinal cells in vitro. Our results support the use of C. elegans as a host model for studying attaching and effacing lesions in vivo, and reveal that the CDK1-formin signal axis is necessary for EHEC-induced microvillar effacement.

Original language	English
Article number	90
Journal	Nature communications
Volume	12
Issue number	1
DOIs	https://doi.org/10.1038/s41467-020-20355-1
Publication status	Published - 2021 Dec

All Science Journal Classification (ASJC) codes

General Chemistry
General Biochemistry,Genetics and Molecular Biology
General Physics and Astronomy

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10.1038/s41467-020-20355-1

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title = "Host CDK-1 and formin mediate microvillar effacement induced by enterohemorrhagic Escherichia coli",

abstract = "Enterohemorrhagic Escherichia coli (EHEC) induces changes to the intestinal cell cytoskeleton and formation of attaching and effacing lesions, characterized by the effacement of microvilli and then formation of actin pedestals to which the bacteria are tightly attached. Here, we use a Caenorhabditis elegans model of EHEC infection to show that microvillar effacement is mediated by a signalling pathway including mitotic cyclin-dependent kinase 1 (CDK1) and diaphanous-related formin 1 (CYK1). Similar observations are also made using EHEC-infected human intestinal cells in vitro. Our results support the use of C. elegans as a host model for studying attaching and effacing lesions in vivo, and reveal that the CDK1-formin signal axis is necessary for EHEC-induced microvillar effacement.",

author = "Huang, {Cheng Rung} and Kuo, {Cheng Ju} and Huang, {Chih Wen} and Chen, {Yu Ting} and Liu, {Bang Yu} and Lee, {Chung Ta} and Chen, {Po Lin} and Chang, {Wen Tsan} and Chen, {Yun Wen} and Lee, {Tzer Min} and Hsieh, {Hui Chen} and Chen, {Chang Shi}",

note = "Publisher Copyright: {\textcopyright} 2021, The Author(s).",

year = "2021",

month = dec,

doi = "10.1038/s41467-020-20355-1",

language = "English",

volume = "12",

journal = "Nature communications",

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TY - JOUR

T1 - Host CDK-1 and formin mediate microvillar effacement induced by enterohemorrhagic Escherichia coli

AU - Huang, Cheng Rung

AU - Kuo, Cheng Ju

AU - Huang, Chih Wen

AU - Chen, Yu Ting

AU - Liu, Bang Yu

AU - Lee, Chung Ta

AU - Chen, Po Lin

AU - Chang, Wen Tsan

AU - Chen, Yun Wen

AU - Lee, Tzer Min

AU - Hsieh, Hui Chen

AU - Chen, Chang Shi

PY - 2021/12

Y1 - 2021/12

N2 - Enterohemorrhagic Escherichia coli (EHEC) induces changes to the intestinal cell cytoskeleton and formation of attaching and effacing lesions, characterized by the effacement of microvilli and then formation of actin pedestals to which the bacteria are tightly attached. Here, we use a Caenorhabditis elegans model of EHEC infection to show that microvillar effacement is mediated by a signalling pathway including mitotic cyclin-dependent kinase 1 (CDK1) and diaphanous-related formin 1 (CYK1). Similar observations are also made using EHEC-infected human intestinal cells in vitro. Our results support the use of C. elegans as a host model for studying attaching and effacing lesions in vivo, and reveal that the CDK1-formin signal axis is necessary for EHEC-induced microvillar effacement.

AB - Enterohemorrhagic Escherichia coli (EHEC) induces changes to the intestinal cell cytoskeleton and formation of attaching and effacing lesions, characterized by the effacement of microvilli and then formation of actin pedestals to which the bacteria are tightly attached. Here, we use a Caenorhabditis elegans model of EHEC infection to show that microvillar effacement is mediated by a signalling pathway including mitotic cyclin-dependent kinase 1 (CDK1) and diaphanous-related formin 1 (CYK1). Similar observations are also made using EHEC-infected human intestinal cells in vitro. Our results support the use of C. elegans as a host model for studying attaching and effacing lesions in vivo, and reveal that the CDK1-formin signal axis is necessary for EHEC-induced microvillar effacement.

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JO - Nature communications

JF - Nature communications

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ER -

Host CDK-1 and formin mediate microvillar effacement induced by enterohemorrhagic Escherichia coli

Abstract

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