Host CDK-1 and formin mediate microvillar effacement induced by enterohemorrhagic Escherichia coli

Cheng Rung Huang, Cheng Ju Kuo, Chih Wen Huang, Yu Ting Chen, Bang Yu Liu, Chung Ta Lee, Po Lin Chen, Wen Tsan Chang, Yun Wen Chen, Tzer Min Lee, Hui Chen Hsieh, Chang Shi Chen

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)

Abstract

Enterohemorrhagic Escherichia coli (EHEC) induces changes to the intestinal cell cytoskeleton and formation of attaching and effacing lesions, characterized by the effacement of microvilli and then formation of actin pedestals to which the bacteria are tightly attached. Here, we use a Caenorhabditis elegans model of EHEC infection to show that microvillar effacement is mediated by a signalling pathway including mitotic cyclin-dependent kinase 1 (CDK1) and diaphanous-related formin 1 (CYK1). Similar observations are also made using EHEC-infected human intestinal cells in vitro. Our results support the use of C. elegans as a host model for studying attaching and effacing lesions in vivo, and reveal that the CDK1-formin signal axis is necessary for EHEC-induced microvillar effacement.

Original languageEnglish
Pages (from-to)90
Number of pages1
JournalNature communications
Volume12
Issue number1
DOIs
Publication statusPublished - 2021 Jan 4

All Science Journal Classification (ASJC) codes

  • Chemistry(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Physics and Astronomy(all)

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