HPLC-fluorescence assay for measuring mosapride in small volumes of rat plasma

Ching Ling Cheng, Ya Win Chang, Chen-Hsi Chou

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

A simple and sensitive HPLC-fluorescence assay was developed for the determination of a gastroprokinetic agent mosapride in small volumes of rat plasma. Samples (50 μL) were treated with 200 μL of the internal standard solution (cisapride, 0.1 μg/mL in acetonitrile). Chromatographic separation was achieved on a C18 column by gradient elution with the mobile phase of acetonitrile-water containing 20 mM potassium dihydrogen phosphate, at a flow rate of 1 mL/min. Fluorescence was measured with excitation and emission set at 315 and 354 nm, respectively. The retention time was about 16 min for cisapride and 20 min for mosapride. No endogenous substances were found to interfere. The calibration curve was linear from 0.015 to 10 μg/mL. The lower limit of quantification was 0.015 μg/mL. The intra- and inter-day precision expressed as relative standard deviation did not exceed 7.7%, and the accuracy was within 4.7% deviation of the nominal concentration. The method was used successfully to investigate the disposition kinetics of mosapride in rats.

Original languageEnglish
Pages (from-to)281-288
Number of pages8
JournalBiomedical Chromatography
Volume24
Issue number3
DOIs
Publication statusPublished - 2010 Mar 1

Fingerprint

Plasma Volume
Cisapride
Rats
Assays
Fluorescence
High Pressure Liquid Chromatography
Plasmas
Calibration
Flow rate
Kinetics
Water
mosapride
acetonitrile

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Biochemistry
  • Molecular Biology
  • Pharmacology
  • Drug Discovery
  • Clinical Biochemistry

Cite this

Cheng, Ching Ling ; Chang, Ya Win ; Chou, Chen-Hsi. / HPLC-fluorescence assay for measuring mosapride in small volumes of rat plasma. In: Biomedical Chromatography. 2010 ; Vol. 24, No. 3. pp. 281-288.
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HPLC-fluorescence assay for measuring mosapride in small volumes of rat plasma. / Cheng, Ching Ling; Chang, Ya Win; Chou, Chen-Hsi.

In: Biomedical Chromatography, Vol. 24, No. 3, 01.03.2010, p. 281-288.

Research output: Contribution to journalArticle

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N2 - A simple and sensitive HPLC-fluorescence assay was developed for the determination of a gastroprokinetic agent mosapride in small volumes of rat plasma. Samples (50 μL) were treated with 200 μL of the internal standard solution (cisapride, 0.1 μg/mL in acetonitrile). Chromatographic separation was achieved on a C18 column by gradient elution with the mobile phase of acetonitrile-water containing 20 mM potassium dihydrogen phosphate, at a flow rate of 1 mL/min. Fluorescence was measured with excitation and emission set at 315 and 354 nm, respectively. The retention time was about 16 min for cisapride and 20 min for mosapride. No endogenous substances were found to interfere. The calibration curve was linear from 0.015 to 10 μg/mL. The lower limit of quantification was 0.015 μg/mL. The intra- and inter-day precision expressed as relative standard deviation did not exceed 7.7%, and the accuracy was within 4.7% deviation of the nominal concentration. The method was used successfully to investigate the disposition kinetics of mosapride in rats.

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