Abstract
A simple and sensitive HPLC-fluorescence assay was developed for the determination of a gastroprokinetic agent mosapride in small volumes of rat plasma. Samples (50 μL) were treated with 200 μL of the internal standard solution (cisapride, 0.1 μg/mL in acetonitrile). Chromatographic separation was achieved on a C18 column by gradient elution with the mobile phase of acetonitrile-water containing 20 mM potassium dihydrogen phosphate, at a flow rate of 1 mL/min. Fluorescence was measured with excitation and emission set at 315 and 354 nm, respectively. The retention time was about 16 min for cisapride and 20 min for mosapride. No endogenous substances were found to interfere. The calibration curve was linear from 0.015 to 10 μg/mL. The lower limit of quantification was 0.015 μg/mL. The intra- and inter-day precision expressed as relative standard deviation did not exceed 7.7%, and the accuracy was within 4.7% deviation of the nominal concentration. The method was used successfully to investigate the disposition kinetics of mosapride in rats.
| Original language | English |
|---|---|
| Pages (from-to) | 281-288 |
| Number of pages | 8 |
| Journal | Biomedical Chromatography |
| Volume | 24 |
| Issue number | 3 |
| DOIs | |
| Publication status | Published - 2010 Mar 1 |
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All Science Journal Classification (ASJC) codes
- Analytical Chemistry
- Biochemistry
- Molecular Biology
- Pharmacology
- Drug Discovery
- Clinical Biochemistry
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HPLC-fluorescence assay for measuring mosapride in small volumes of rat plasma. / Cheng, Ching Ling; Chang, Ya Win; Chou, Chen-Hsi.
In: Biomedical Chromatography, Vol. 24, No. 3, 01.03.2010, p. 281-288.Research output: Contribution to journal › Article
TY - JOUR
T1 - HPLC-fluorescence assay for measuring mosapride in small volumes of rat plasma
AU - Cheng, Ching Ling
AU - Chang, Ya Win
AU - Chou, Chen-Hsi
PY - 2010/3/1
Y1 - 2010/3/1
N2 - A simple and sensitive HPLC-fluorescence assay was developed for the determination of a gastroprokinetic agent mosapride in small volumes of rat plasma. Samples (50 μL) were treated with 200 μL of the internal standard solution (cisapride, 0.1 μg/mL in acetonitrile). Chromatographic separation was achieved on a C18 column by gradient elution with the mobile phase of acetonitrile-water containing 20 mM potassium dihydrogen phosphate, at a flow rate of 1 mL/min. Fluorescence was measured with excitation and emission set at 315 and 354 nm, respectively. The retention time was about 16 min for cisapride and 20 min for mosapride. No endogenous substances were found to interfere. The calibration curve was linear from 0.015 to 10 μg/mL. The lower limit of quantification was 0.015 μg/mL. The intra- and inter-day precision expressed as relative standard deviation did not exceed 7.7%, and the accuracy was within 4.7% deviation of the nominal concentration. The method was used successfully to investigate the disposition kinetics of mosapride in rats.
AB - A simple and sensitive HPLC-fluorescence assay was developed for the determination of a gastroprokinetic agent mosapride in small volumes of rat plasma. Samples (50 μL) were treated with 200 μL of the internal standard solution (cisapride, 0.1 μg/mL in acetonitrile). Chromatographic separation was achieved on a C18 column by gradient elution with the mobile phase of acetonitrile-water containing 20 mM potassium dihydrogen phosphate, at a flow rate of 1 mL/min. Fluorescence was measured with excitation and emission set at 315 and 354 nm, respectively. The retention time was about 16 min for cisapride and 20 min for mosapride. No endogenous substances were found to interfere. The calibration curve was linear from 0.015 to 10 μg/mL. The lower limit of quantification was 0.015 μg/mL. The intra- and inter-day precision expressed as relative standard deviation did not exceed 7.7%, and the accuracy was within 4.7% deviation of the nominal concentration. The method was used successfully to investigate the disposition kinetics of mosapride in rats.
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U2 - 10.1002/bmc.1285
DO - 10.1002/bmc.1285
M3 - Article
VL - 24
SP - 281
EP - 288
JO - Biomedical Chromatography
JF - Biomedical Chromatography
SN - 0269-3879
IS - 3
ER -