Human sebum extract induces barrier disruption and cytokine expression in murine epidermis

Jiun Wen Guo, Tzu Kai Lin, Chin Han Wu, Kai Che Wei, Cheng Che E. Lan, Amy Chia Ying Peng, Jui-Chen Tsai, Hamm Ming Sheu

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Background: Previous studies have shown that human sebum may play a role in barrier function but with much debate. Objective: To elucidate the effects of human sebum on skin barrier function. Methods: We used hairless mouse skin to study the functional and morphological alternation of epidermis after the application of human sebum. Results: The results showed a significant increase in transepidermal water loss and erythema value, and a decrease in skin hydration, accompanied by epidermal hyperplasia with parakeratosis following sebum application. Nile red staining together with electron microscopic examination confirmed the underlying mechanisms for sebum-induced barrier disruption are related directly to the interaction of sebum with the intracellular lipid lamellae of the SC, thereby leading to the increase in the fluidity of SC intracellular lipids as demonstrated by ATR-FTIR measurement. An inflammatory reaction characterized by an enhanced cytokine cascade, including up-regulation of TNF-α, IL-1α and IL-6, was also observed. On the other hand, there were insignificant expression of thymic stromal lymphopoietin and unchanged serum levels of IgE, suggesting non-immunogenic stimulation by sebum treatment. Conclusion: It may be concluded that inflammation induced by excess amount of sebum is more likely an irritant contact dermatitis rather than an allergic one. Moreover, these findings implicated possible relationships between sebum, irritant contact dermatitis, and seborrheic dermatitis.

Original languageEnglish
Pages (from-to)34-43
Number of pages10
JournalJournal of Dermatological Science
Volume78
Issue number1
DOIs
Publication statusPublished - 2015 Apr 1

Fingerprint

Dermatitis
Sebum
Epidermis
Skin
Irritants
Cytokines
Lipids
Fluidity
Interleukin-1
Hydration
Irritant Dermatitis
Immunoglobulin E
Interleukin-6
Microscopic examination
Contact Dermatitis
Electrons
Water
Parakeratosis
Seborrheic Dermatitis
Hairless Mouse

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Dermatology

Cite this

Guo, J. W., Lin, T. K., Wu, C. H., Wei, K. C., Lan, C. C. E., Peng, A. C. Y., ... Sheu, H. M. (2015). Human sebum extract induces barrier disruption and cytokine expression in murine epidermis. Journal of Dermatological Science, 78(1), 34-43. https://doi.org/10.1016/j.jdermsci.2015.01.010
Guo, Jiun Wen ; Lin, Tzu Kai ; Wu, Chin Han ; Wei, Kai Che ; Lan, Cheng Che E. ; Peng, Amy Chia Ying ; Tsai, Jui-Chen ; Sheu, Hamm Ming. / Human sebum extract induces barrier disruption and cytokine expression in murine epidermis. In: Journal of Dermatological Science. 2015 ; Vol. 78, No. 1. pp. 34-43.
@article{d3b27186460f4d5cb119ae62a8c7e555,
title = "Human sebum extract induces barrier disruption and cytokine expression in murine epidermis",
abstract = "Background: Previous studies have shown that human sebum may play a role in barrier function but with much debate. Objective: To elucidate the effects of human sebum on skin barrier function. Methods: We used hairless mouse skin to study the functional and morphological alternation of epidermis after the application of human sebum. Results: The results showed a significant increase in transepidermal water loss and erythema value, and a decrease in skin hydration, accompanied by epidermal hyperplasia with parakeratosis following sebum application. Nile red staining together with electron microscopic examination confirmed the underlying mechanisms for sebum-induced barrier disruption are related directly to the interaction of sebum with the intracellular lipid lamellae of the SC, thereby leading to the increase in the fluidity of SC intracellular lipids as demonstrated by ATR-FTIR measurement. An inflammatory reaction characterized by an enhanced cytokine cascade, including up-regulation of TNF-α, IL-1α and IL-6, was also observed. On the other hand, there were insignificant expression of thymic stromal lymphopoietin and unchanged serum levels of IgE, suggesting non-immunogenic stimulation by sebum treatment. Conclusion: It may be concluded that inflammation induced by excess amount of sebum is more likely an irritant contact dermatitis rather than an allergic one. Moreover, these findings implicated possible relationships between sebum, irritant contact dermatitis, and seborrheic dermatitis.",
author = "Guo, {Jiun Wen} and Lin, {Tzu Kai} and Wu, {Chin Han} and Wei, {Kai Che} and Lan, {Cheng Che E.} and Peng, {Amy Chia Ying} and Jui-Chen Tsai and Sheu, {Hamm Ming}",
year = "2015",
month = "4",
day = "1",
doi = "10.1016/j.jdermsci.2015.01.010",
language = "English",
volume = "78",
pages = "34--43",
journal = "Journal of Dermatological Science",
issn = "0923-1811",
publisher = "Elsevier Ireland Ltd",
number = "1",

}

Human sebum extract induces barrier disruption and cytokine expression in murine epidermis. / Guo, Jiun Wen; Lin, Tzu Kai; Wu, Chin Han; Wei, Kai Che; Lan, Cheng Che E.; Peng, Amy Chia Ying; Tsai, Jui-Chen; Sheu, Hamm Ming.

In: Journal of Dermatological Science, Vol. 78, No. 1, 01.04.2015, p. 34-43.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Human sebum extract induces barrier disruption and cytokine expression in murine epidermis

AU - Guo, Jiun Wen

AU - Lin, Tzu Kai

AU - Wu, Chin Han

AU - Wei, Kai Che

AU - Lan, Cheng Che E.

AU - Peng, Amy Chia Ying

AU - Tsai, Jui-Chen

AU - Sheu, Hamm Ming

PY - 2015/4/1

Y1 - 2015/4/1

N2 - Background: Previous studies have shown that human sebum may play a role in barrier function but with much debate. Objective: To elucidate the effects of human sebum on skin barrier function. Methods: We used hairless mouse skin to study the functional and morphological alternation of epidermis after the application of human sebum. Results: The results showed a significant increase in transepidermal water loss and erythema value, and a decrease in skin hydration, accompanied by epidermal hyperplasia with parakeratosis following sebum application. Nile red staining together with electron microscopic examination confirmed the underlying mechanisms for sebum-induced barrier disruption are related directly to the interaction of sebum with the intracellular lipid lamellae of the SC, thereby leading to the increase in the fluidity of SC intracellular lipids as demonstrated by ATR-FTIR measurement. An inflammatory reaction characterized by an enhanced cytokine cascade, including up-regulation of TNF-α, IL-1α and IL-6, was also observed. On the other hand, there were insignificant expression of thymic stromal lymphopoietin and unchanged serum levels of IgE, suggesting non-immunogenic stimulation by sebum treatment. Conclusion: It may be concluded that inflammation induced by excess amount of sebum is more likely an irritant contact dermatitis rather than an allergic one. Moreover, these findings implicated possible relationships between sebum, irritant contact dermatitis, and seborrheic dermatitis.

AB - Background: Previous studies have shown that human sebum may play a role in barrier function but with much debate. Objective: To elucidate the effects of human sebum on skin barrier function. Methods: We used hairless mouse skin to study the functional and morphological alternation of epidermis after the application of human sebum. Results: The results showed a significant increase in transepidermal water loss and erythema value, and a decrease in skin hydration, accompanied by epidermal hyperplasia with parakeratosis following sebum application. Nile red staining together with electron microscopic examination confirmed the underlying mechanisms for sebum-induced barrier disruption are related directly to the interaction of sebum with the intracellular lipid lamellae of the SC, thereby leading to the increase in the fluidity of SC intracellular lipids as demonstrated by ATR-FTIR measurement. An inflammatory reaction characterized by an enhanced cytokine cascade, including up-regulation of TNF-α, IL-1α and IL-6, was also observed. On the other hand, there were insignificant expression of thymic stromal lymphopoietin and unchanged serum levels of IgE, suggesting non-immunogenic stimulation by sebum treatment. Conclusion: It may be concluded that inflammation induced by excess amount of sebum is more likely an irritant contact dermatitis rather than an allergic one. Moreover, these findings implicated possible relationships between sebum, irritant contact dermatitis, and seborrheic dermatitis.

UR - http://www.scopus.com/inward/record.url?scp=84925945077&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84925945077&partnerID=8YFLogxK

U2 - 10.1016/j.jdermsci.2015.01.010

DO - 10.1016/j.jdermsci.2015.01.010

M3 - Article

VL - 78

SP - 34

EP - 43

JO - Journal of Dermatological Science

JF - Journal of Dermatological Science

SN - 0923-1811

IS - 1

ER -