Abstract
Background: Previous studies have shown that human sebum may play a role in barrier function but with much debate. Objective: To elucidate the effects of human sebum on skin barrier function. Methods: We used hairless mouse skin to study the functional and morphological alternation of epidermis after the application of human sebum. Results: The results showed a significant increase in transepidermal water loss and erythema value, and a decrease in skin hydration, accompanied by epidermal hyperplasia with parakeratosis following sebum application. Nile red staining together with electron microscopic examination confirmed the underlying mechanisms for sebum-induced barrier disruption are related directly to the interaction of sebum with the intracellular lipid lamellae of the SC, thereby leading to the increase in the fluidity of SC intracellular lipids as demonstrated by ATR-FTIR measurement. An inflammatory reaction characterized by an enhanced cytokine cascade, including up-regulation of TNF-α, IL-1α and IL-6, was also observed. On the other hand, there were insignificant expression of thymic stromal lymphopoietin and unchanged serum levels of IgE, suggesting non-immunogenic stimulation by sebum treatment. Conclusion: It may be concluded that inflammation induced by excess amount of sebum is more likely an irritant contact dermatitis rather than an allergic one. Moreover, these findings implicated possible relationships between sebum, irritant contact dermatitis, and seborrheic dermatitis.
| Original language | English |
|---|---|
| Pages (from-to) | 34-43 |
| Number of pages | 10 |
| Journal | Journal of Dermatological Science |
| Volume | 78 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - 2015 Apr 1 |
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All Science Journal Classification (ASJC) codes
- Biochemistry
- Molecular Biology
- Dermatology
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Human sebum extract induces barrier disruption and cytokine expression in murine epidermis. / Guo, Jiun Wen; Lin, Tzu Kai; Wu, Chin Han; Wei, Kai Che; Lan, Cheng Che E.; Peng, Amy Chia Ying; Tsai, Jui-Chen; Sheu, Hamm Ming.
In: Journal of Dermatological Science, Vol. 78, No. 1, 01.04.2015, p. 34-43.Research output: Contribution to journal › Article
TY - JOUR
T1 - Human sebum extract induces barrier disruption and cytokine expression in murine epidermis
AU - Guo, Jiun Wen
AU - Lin, Tzu Kai
AU - Wu, Chin Han
AU - Wei, Kai Che
AU - Lan, Cheng Che E.
AU - Peng, Amy Chia Ying
AU - Tsai, Jui-Chen
AU - Sheu, Hamm Ming
PY - 2015/4/1
Y1 - 2015/4/1
N2 - Background: Previous studies have shown that human sebum may play a role in barrier function but with much debate. Objective: To elucidate the effects of human sebum on skin barrier function. Methods: We used hairless mouse skin to study the functional and morphological alternation of epidermis after the application of human sebum. Results: The results showed a significant increase in transepidermal water loss and erythema value, and a decrease in skin hydration, accompanied by epidermal hyperplasia with parakeratosis following sebum application. Nile red staining together with electron microscopic examination confirmed the underlying mechanisms for sebum-induced barrier disruption are related directly to the interaction of sebum with the intracellular lipid lamellae of the SC, thereby leading to the increase in the fluidity of SC intracellular lipids as demonstrated by ATR-FTIR measurement. An inflammatory reaction characterized by an enhanced cytokine cascade, including up-regulation of TNF-α, IL-1α and IL-6, was also observed. On the other hand, there were insignificant expression of thymic stromal lymphopoietin and unchanged serum levels of IgE, suggesting non-immunogenic stimulation by sebum treatment. Conclusion: It may be concluded that inflammation induced by excess amount of sebum is more likely an irritant contact dermatitis rather than an allergic one. Moreover, these findings implicated possible relationships between sebum, irritant contact dermatitis, and seborrheic dermatitis.
AB - Background: Previous studies have shown that human sebum may play a role in barrier function but with much debate. Objective: To elucidate the effects of human sebum on skin barrier function. Methods: We used hairless mouse skin to study the functional and morphological alternation of epidermis after the application of human sebum. Results: The results showed a significant increase in transepidermal water loss and erythema value, and a decrease in skin hydration, accompanied by epidermal hyperplasia with parakeratosis following sebum application. Nile red staining together with electron microscopic examination confirmed the underlying mechanisms for sebum-induced barrier disruption are related directly to the interaction of sebum with the intracellular lipid lamellae of the SC, thereby leading to the increase in the fluidity of SC intracellular lipids as demonstrated by ATR-FTIR measurement. An inflammatory reaction characterized by an enhanced cytokine cascade, including up-regulation of TNF-α, IL-1α and IL-6, was also observed. On the other hand, there were insignificant expression of thymic stromal lymphopoietin and unchanged serum levels of IgE, suggesting non-immunogenic stimulation by sebum treatment. Conclusion: It may be concluded that inflammation induced by excess amount of sebum is more likely an irritant contact dermatitis rather than an allergic one. Moreover, these findings implicated possible relationships between sebum, irritant contact dermatitis, and seborrheic dermatitis.
UR - http://www.scopus.com/inward/record.url?scp=84925945077&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84925945077&partnerID=8YFLogxK
U2 - 10.1016/j.jdermsci.2015.01.010
DO - 10.1016/j.jdermsci.2015.01.010
M3 - Article
C2 - 25680851
AN - SCOPUS:84925945077
VL - 78
SP - 34
EP - 43
JO - Journal of Dermatological Science
JF - Journal of Dermatological Science
SN - 0923-1811
IS - 1
ER -