Abstract
We examined, in vitro, whether hyaluronan induces slow cycling in placenta-derived mesenchymal stem cells (PDMSCs) by comparing cell growth on a hyaluronan-coated surface with cell growth on a tissue-culture polystyrene surface. The hyaluronan-coated surface significantly downregulated the proliferation of PDMSCs, more of which were maintained in the G 0/G1 phases than were cells on the tissue-culture polystyrene surface. Both PKH-26 labeling and BrdU incorporation assays showed that most PDMSCs grown on a hyaluronan-coated surface duplicated during cultivation indicating that the hyaluronan-coated surface did not inhibit PDMSCs from entering the cell cycle. Mitotic synchronization showed that the G 1-phase transit was prolonged in PDMSCs growing on a hyaluronan-coated surface. Increases in p27Kip1 and p130 were the crucial factors that allowed hyaluronan to lengthen the G1 phase. Thus, hyaluronan might be a promising candidate for maintaining stem cells in slow-cycling mode by prolonging their G1-phase transit.
Original language | English |
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Pages (from-to) | 435-443 |
Number of pages | 9 |
Journal | Cell and Tissue Research |
Volume | 334 |
Issue number | 3 |
DOIs | |
Publication status | Published - 2008 Dec |
All Science Journal Classification (ASJC) codes
- Pathology and Forensic Medicine
- Histology
- Cell Biology