TY - JOUR
T1 - Hyaluronic acid-complement interactions-II. Role of divalent cations and gelatin
AU - Nan-Shan, Chang
AU - Boackle, Robert J.
N1 - Funding Information:
*Publication No. 707 from the Department of Basic and Clinical Immunology and Microbiology, Medical Uni-versity of South Carolina. Research supported in part by NIH DE-05049. tcorrespondence should be addressed to this author.
PY - 1985/8
Y1 - 1985/8
N2 - Native hyaluronic acid (HA) is reported to be a weak anticomplementary agent. However, the normal buffer systems used for complement tests incorporate gelatin, Ca2+ and Mg2+, which may bind to HA, influence its conformation and interfere with its anticomplementary reactions with complement components such as Cl. In this study, metal ions (Ca2+ and Mg2+), gelatin and fibronectin appeared to react with native HA preparations and block their anticomplementary effects on Cl. In previous studies, we obtained evidence for a relationship between reversible heat-induced HA conformational changes and a subsequent reversible increase in anticomplementary activity. The anticomplementary activity of heat-treated HA preparations was also reduced by gelatin.
AB - Native hyaluronic acid (HA) is reported to be a weak anticomplementary agent. However, the normal buffer systems used for complement tests incorporate gelatin, Ca2+ and Mg2+, which may bind to HA, influence its conformation and interfere with its anticomplementary reactions with complement components such as Cl. In this study, metal ions (Ca2+ and Mg2+), gelatin and fibronectin appeared to react with native HA preparations and block their anticomplementary effects on Cl. In previous studies, we obtained evidence for a relationship between reversible heat-induced HA conformational changes and a subsequent reversible increase in anticomplementary activity. The anticomplementary activity of heat-treated HA preparations was also reduced by gelatin.
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U2 - 10.1016/0161-5890(85)90068-9
DO - 10.1016/0161-5890(85)90068-9
M3 - Article
C2 - 4047042
AN - SCOPUS:0021971830
SN - 0161-5890
VL - 22
SP - 843
EP - 848
JO - Molecular Immunology
JF - Molecular Immunology
IS - 8
ER -