Hybrid liquid biphasic system for cell disruption and simultaneous lipid extraction from microalgae Chlorella sorokiniana CY-1 for biofuel production

Guo Yong Yew, Kit Wayne Chew, Marlinda Abdul Malek, Yeek Chia Ho, Wei Hsin Chen, Tau Chuan Ling, Pau Loke Show

Research output: Contribution to journalArticle

Abstract

Background: The extraction of lipids from microalgae requires a pretreatment process to break the cell wall and subsequent extraction processes to obtain the lipids for biofuels production. The multistep operation tends to incur high costs and are energy intensive due to longer process operations. This research work applies the combination of radicals from hydrogen peroxide with an organic solvent as a chemical pretreatment method for disrupting the cell wall of microalgae and simultaneously extracting lipids from the biomass in a one-step biphasic solution. Result: Several parameters which can affect the biphasic system were analyzed: contact time, volume of solvent, volume ratio, type of organic solvent, biomass amount and concentration of solvents, to extract the highest amount of lipids from microalgae. The results were optimized and up to 83.5% of lipid recovery yield and 94.6% of enhancement was successfully achieved. The results obtain from GC-FID were similar to the analysis of triglyceride lipid standard. Conclusion: The profound hybrid biphasic system shows great potential to radically disrupt the cell wall of microalgae and instantaneously extract lipids in a single-step approach. The lipids extracted were tested to for its comparability to biodiesel performance.

Original languageEnglish
Article number252
JournalBiotechnology for Biofuels
Volume12
Issue number1
DOIs
Publication statusPublished - 2019 Oct 25

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Microalgae
Chlorella
Biofuels
biofuel
Lipids
lipid
liquid
Liquids
Cell Wall
Cells
Biomass
Organic solvents
chemical method
biomass
research work
Biodiesel
Hybrid systems
Hydrogen peroxide
hydrogen peroxide
Hydrogen Peroxide

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Applied Microbiology and Biotechnology
  • Renewable Energy, Sustainability and the Environment
  • Energy(all)
  • Management, Monitoring, Policy and Law

Cite this

Yew, Guo Yong ; Chew, Kit Wayne ; Malek, Marlinda Abdul ; Ho, Yeek Chia ; Chen, Wei Hsin ; Ling, Tau Chuan ; Show, Pau Loke. / Hybrid liquid biphasic system for cell disruption and simultaneous lipid extraction from microalgae Chlorella sorokiniana CY-1 for biofuel production. In: Biotechnology for Biofuels. 2019 ; Vol. 12, No. 1.
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Hybrid liquid biphasic system for cell disruption and simultaneous lipid extraction from microalgae Chlorella sorokiniana CY-1 for biofuel production. / Yew, Guo Yong; Chew, Kit Wayne; Malek, Marlinda Abdul; Ho, Yeek Chia; Chen, Wei Hsin; Ling, Tau Chuan; Show, Pau Loke.

In: Biotechnology for Biofuels, Vol. 12, No. 1, 252, 25.10.2019.

Research output: Contribution to journalArticle

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