Hypoxic-ischemic retinal injury in rat pups

Hsiu Mei Huang, Chao-Ching Huang, Pi Lien Hung, Ying Chao Chang

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Background:Visual loss associated with brain damage, especially hypoxic-ischemic (HI) encephalopathy, is the most common cause of visual impairment in children in developed countries. We hypothesized that HI insults can cause long-term damage in immature eyes.Methods:In postnatal day 7 rat pups, HI was induced by unilateral common carotid artery ligation followed by hypoxia. Retina damage was assessed by electroretinography (ERG) and cell counting. Neuronal injury and astrogliosis were evaluated by terminal deoxynucleotidyl transferase nick-end labeling, cleaved caspase 3, ED1, and glial fibrillary acidic protein immunostaining.Results:We observed rapid and persistently extensive injuries in the ganglia cell layer (GCL), inner plexiform layer, and inner nuclear layer (INL) in ipsilateral retinas after HI injury, corresponding to the marked alteration in ERG. HI insult caused prominent microglial and Mller cell activation in ipsilateral inner retinas. Neuronal death in the GCL and INL after HI injury was mainly apoptotic, involving caspase-dependent pathways.Conclusion:Our study demonstrated the first evidence of HI retinal damage at both the pathological and functional level using the Vannucci model in neonatal rats. Because retinal damage is often associated with HI injury, it is important to demonstrate that a particular neuroprotective strategy effectively preserves the retina in addition to the brain.

Original languageEnglish
Pages (from-to)224-231
Number of pages8
JournalPediatric Research
Volume72
Issue number3
DOIs
Publication statusPublished - 2012 Sep 1

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Retina
Wounds and Injuries
Electroretinography
Ganglia
Brain Hypoxia-Ischemia
DNA Nucleotidylexotransferase
Common Carotid Artery
Glial Fibrillary Acidic Protein
Vision Disorders
Brain
Caspases
Developed Countries
Caspase 3
Ligation

All Science Journal Classification (ASJC) codes

  • Pediatrics, Perinatology, and Child Health

Cite this

Huang, Hsiu Mei ; Huang, Chao-Ching ; Hung, Pi Lien ; Chang, Ying Chao. / Hypoxic-ischemic retinal injury in rat pups. In: Pediatric Research. 2012 ; Vol. 72, No. 3. pp. 224-231.
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Huang, HM, Huang, C-C, Hung, PL & Chang, YC 2012, 'Hypoxic-ischemic retinal injury in rat pups', Pediatric Research, vol. 72, no. 3, pp. 224-231. https://doi.org/10.1038/pr.2012.74

Hypoxic-ischemic retinal injury in rat pups. / Huang, Hsiu Mei; Huang, Chao-Ching; Hung, Pi Lien; Chang, Ying Chao.

In: Pediatric Research, Vol. 72, No. 3, 01.09.2012, p. 224-231.

Research output: Contribution to journalArticle

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